PXD046219-2

PXD046219 is an original dataset announced via ProteomeXchange.

Title	Differential Protein Expression and Metabolite Profiling in Glaucoma: Insights from a Multi-Omics Analysis
Description	Glaucoma is a group of diseases characterized by progressive optic nerve damage leading to irreversible visual field loss, often associated with elevated intraocular pressure (IOP). However, the specific underlying factors or molecular mechanisms distinguishing various glaucoma subtypes are still not fully understood. In this study, we enrolled a total of 27 subjects, comprising 5 exfoliation syndrome (XFS), 4 exfoliation glaucoma (XFG), 11 primary open-angle glaucoma (POAG), and 7 cataract patients (Control) for a comparative multi-omics analysis including both proteomics and metabolomics of the aqueous humor (AH). The AH proteome and metabolome were analyzed separately using data-independent acquisition (DIA) and data-dependent acquisition (DDA) methods, after which the results were additionally integrated for a comprehensive analysis. The comparative proteomics analysis led to the identification of differentially expressed proteins (DEPs) related to lipid metabolism, complement activation, and extracellular matrix (ECM) regulation, which were commonly up-regulated in both XFG and POAG groups. The metabolomics analysis revealed differentially expressed metabolites (DEMs) particularly those involved in amino acids, suggesting their role in antioxidative processes. Thus, bioinformatic analysis suggested significant substances, such as VTN, APOA1, C6, and L-phenylalanine, that showed quantitative alterations in glaucoma patients. In addition, the integrating the individual omics analyses revealed interaction networks involving PLG, APOA1, and L-phenylalanine, or C3, APOD, and L-valine, suggesting the quantitative relationship in protein-metabolite interactions related to inflammation and lipid metabolism within the AH of glaucoma patients. Furthermore, the correlation approach identified a relationship between FGG and L-phenylalanine, and VTN and inosine, implying potential interactions among compounds not previously identified. These findings would provide valuable insights into the complex molecular mechanism differentiating glaucoma subtypes and may benefit further research into the clinical application of AH proteome and metabolome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:49.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeong-hun Mok
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-18 16:05:21	ID requested
1	2024-04-11 05:52:02	announced
⏵ 2	2024-10-22 06:35:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, multi-omics, metabolomics,Pseudoexfoliation, primary open-angle glaucoma, extracellular matrix

Jong Chul Han
contact affiliation	Department of Medical Device Management and Research, SAIHST, Sungkyunkwan University, Seoul 06355, Korea
contact email	heartmedic79@gmail.com
lab head
Jeong-hun Mok
contact affiliation	Gachon University Gil Medical Center, Incheon, Republic of Korea
contact email	jeonghunmok@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046219
     1. Label: PRIDE project
     2. Name: Differential Protein Expression and Metabolite Profiling in Glaucoma: Insights from a Multi-Omics Analysis