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PXD046197-1

PXD046197 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe analysis of RhMYB73-interacted proteins in rose by LC-MS
DescriptionThe size of plant lateral organs is determined by well-coordinated cell proliferation and cell expansion. Here, we report that miR159, an evolutionarily conserved microRNA, plays an essential role in regulating cell division in rose (Rosa hybrida) petals by modulating cytokinin catabolism. We uncovered that Cytokinin Oxidase/Dehydrogenase6 (CKX6) is a target of miR159 in petals. Knocking down miR159 levels resulted in the accumulation of CKX6 transcripts and earlier cytokinin clearance, leading to a shortened cell division period and smaller petals. Conversely, knocking down CKX6 caused cytokinin accumulation and a prolonged developmental cell division period, mimicking the effects of exogenous cytokinin application. MYB73, a R2R3-type MYB transcription repressor, recruited a co-repressor (TOPLESS) and a histone deacetylase (HDA19) to form a suppression complex, which regulated MIR159 expression by modulating histone H3 lysine 9 acetylation levels at the MIR159 promoter. Our work sheds light on mechanisms for ensuring the correct timing of the exit from the cell division phase and thus organ size regulation by controlling cytokinin catabolism.
HostingRepositoryiProX
AnnounceDate2023-10-18
AnnouncementXMLSubmission_2023-10-18_20:02:38.092.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWeikun Jing
SpeciesList scientific name: Rosa hybrid cultivar; NCBI TaxID: 128735;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-17 19:42:02ID requested
12023-10-18 20:02:38announced
22023-12-04 20:55:42announced2023-12-05: Update publication information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plant, Rose, Petal, RhMYB73, MS
Contact List
Nan Ma
contact affiliationChina Agricultural University
contact emailma_nan@cau.edu.cn
lab head
Weikun Jing
contact affiliationChina Agricultural University
contact emailjwk093@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI