PXD046197-1
PXD046197 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The analysis of RhMYB73-interacted proteins in rose by LC-MS |
| Description | The size of plant lateral organs is determined by well-coordinated cell proliferation and cell expansion. Here, we report that miR159, an evolutionarily conserved microRNA, plays an essential role in regulating cell division in rose (Rosa hybrida) petals by modulating cytokinin catabolism. We uncovered that Cytokinin Oxidase/Dehydrogenase6 (CKX6) is a target of miR159 in petals. Knocking down miR159 levels resulted in the accumulation of CKX6 transcripts and earlier cytokinin clearance, leading to a shortened cell division period and smaller petals. Conversely, knocking down CKX6 caused cytokinin accumulation and a prolonged developmental cell division period, mimicking the effects of exogenous cytokinin application. MYB73, a R2R3-type MYB transcription repressor, recruited a co-repressor (TOPLESS) and a histone deacetylase (HDA19) to form a suppression complex, which regulated MIR159 expression by modulating histone H3 lysine 9 acetylation levels at the MIR159 promoter. Our work sheds light on mechanisms for ensuring the correct timing of the exit from the cell division phase and thus organ size regulation by controlling cytokinin catabolism. |
| HostingRepository | iProX |
| AnnounceDate | 2023-10-18 |
| AnnouncementXML | Submission_2023-10-18_20:02:38.092.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Weikun Jing |
| SpeciesList | scientific name: Rosa hybrid cultivar; NCBI TaxID: 128735; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-17 19:42:02 | ID requested | |
| ⏵ 1 | 2023-10-18 20:02:38 | announced | |
| 2 | 2023-12-04 20:55:42 | announced | 2023-12-05: Update publication information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Plant, Rose, Petal, RhMYB73, MS |
Contact List
| Nan Ma | |
|---|---|
| contact affiliation | China Agricultural University |
| contact email | ma_nan@cau.edu.cn |
| lab head | |
| Weikun Jing | |
| contact affiliation | China Agricultural University |
| contact email | jwk093@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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