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PXD046170-1
PXD046170 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Sperm and seminal plasma proteoform atlas in bulls with contrasting semen freezability: A story deciphered by top-down mass spectrometry |
| Description | Top-down proteomics was employed to construct proteoform atlas of sperm and seminal plasma from bulls with low (LF) and high (HF) semen freezability. Sperm and seminal proteins were fractionated by tandem size exclusion chromatography (< 30 kDa) and analyzed by reversed-phase liquid chromatography-tandem mass spectrometry. This approach enabled the identification of 299 seminal plasma (from 46 proteoform families) and 267 sperm proteoforms (from 139 families). Seventy proteoforms belonging to beta-defensin 10, NPPC, caltrin, seminal ribonuclease, osteopontin, and BSP3 families defined the seminal plasma signature of HF bulls. On the other hand, LF seminal plasma exhibited a profile with 77 proteoforms, including those from caltrin, NPPC, osteopontin, BSP3, serpin family A member 5, and B-NGF families. Proteoform families of HF and LF bulls were associated with Ca2+ uptake, sperm capacitation, acrosome reaction, sperm protection, fertilization and proteolytic processes. Proteoforms upregulated (33) in HF sperm included members of NPPC, caltrin, and cylicin-2 families; while proteoforms (22) of caltrin, cylicin-2, ATP synthases, malate dehydrogenase families were among those upregulated in LF sperm. Truncated and acetylated histone H2A and non-truncated and acetylated c-Myc binding protein were prevalent in LF sperm, cylicin-2 proteoforms in HF and LF sperm and truncated glyceraldehyde-3-phosphate dehydrogenases in HF sperm. In silico analysis indicated the enrichment of mitochondrial metabolic pathways in HF sperm, including fatty acid metabolism and TCA cycle. Conclusively, our study brings unprecedented information about the proteoforms of bovine seminal plasma and sperm. Post-translational processing appears to define bio-properties of semen proteins and their associations with sperm cryoresistance. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-31 |
| AnnouncementXML | Submission_2026-05-31_15:40:29.884.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Arabela Guedes de Azevedo Viana |
| SpeciesList | scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913; |
| ModificationList | Acetyl; half cystine; Phospho; Gln->pyro-Glu |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-16 20:15:29 | ID requested | |
| ⏵ 1 | 2026-05-31 15:40:30 | announced |
Publication List
| Guedes de Azevedo Viana A, Gomes FP, Kaya A, Diedrich J, Topper E, Di Silvestre D, Machado-Neves M, Memili E, Moura AA, Yates JR, Seminal proteoforms from bulls with contrasting semen freezability: a story deciphered by top-down mass spectrometry. Reproduction, 169(2):(2025) [pubmed] |
Keyword List
| submitter keyword: sperm, seminal plasma, proteoforms, freezability |
Contact List
| John Yates | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | jyates@scripps.ed |
| lab head | |
| Arabela Guedes de Azevedo Viana | |
| contact affiliation | Federal University of Vi?osa |
| contact email | arabela.viana@ufv.br |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000093128/ |
