PXD046167-1

PXD046167 is an original dataset announced via ProteomeXchange.

Title	Protein profile of purified plasma and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis
Description	To characterize extracellular vesicles proteins cargo from the aqueous humor and plasma of patients with ocular toxoplasmosis. Methods: Aqueous humor (AH) and plasma were collected from 6 patients with active ocular toxoplasmosis (OT) and 6 cataract patients (CAT). Extracellular vesicles (EVs) were isolated, and western blotting and mass spectrometry were performed for protein analysis. Results: All plasma samples from OT and CAT patients were positive for tetraspanins CD63 and TSG101 markers. However, only the AH from OT patients were positive for CD63. A total of 67 new proteins not previously reported in EVs were found in the AH and plasma from OT and CAT groups. Among these, 10 and 7 proteins were found only in the samples from CAT and OT groups, respectively. In general, these proteins were involved in immune system activation, retina homeostasis, factors related to infections and retina-associated disease. Conclusion: The distinct protein signatures between OT and CAT patients may be helpful in the differential diagnosis of OT. However, more studies are needed to better understand the role of these proteins in the pathogenesis of OT.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_18:02:09.834.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohamed Abdouh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-16 13:46:50	ID requested
⏵ 1	2025-11-09 18:02:12	announced

10.5935/0004-2749.2023-0037;

Costa DF, Pessuti CL, Tsering T, Abdouh M, Ribeiro K, Nascimento H, Commodaro AG, Burnier JV, Belfort R, Burnier MN, Protein profile of purified plasma- and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis. Arq Bras Oftalmol, 88(1):e20230037(2024) [pubmed]

submitter keyword: Extracellular Vesicles, Aqueous Humor

Plasma, Liquid Biopsy, Proteomics, Toxoplasma gondii, Ocular Toxoplasmosis

Julia Burnier
contact affiliation	Scientist, IR-MUHC, Glen site Cancer Research Program Center for Translational Biology Assistant Professor, Gerald Bronfman Department of Oncology, Faculty of Medicine and Health Sciences, McGill University
contact email	julia.burnier@mcgill.ca
lab head
Mohamed Abdouh
contact affiliation	Research Institute of the McGill University Health Centre
contact email	abdouhm2009@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046167
     1. Label: PRIDE project
     2. Name: Protein profile of purified plasma and aqueous humor-derived extracellular vesicles from patients with ocular toxoplasmosis