PXD046156-1

PXD046156 is an original dataset announced via ProteomeXchange.

Title	Evolution of genotypic and phenotypic diversity in multispecies biofilms
Description	Interactions occurring in microbial communities can affect the fitness and adaptability of their individual members when facing changing environmental conditions. This study investigated the impact of interspecies interactions in selecting Bacillus thuringiensis variants emerging in biofilm and planktonic environments. During evolution experiments, a B. thuringiensis distinct phenotypic variant of B. thuringiensis frequently occurred, despite the presence of other species or culture setup. Remarkably, selection of this variant was significantly favored over its ancestor in biofilm settings and when coexisting with other species co-isolated from a wastewater facility, namely Pseudomonas defluvii and Pseudomonas brenneri. Interestingly, the evolved phenotype did not show higher biofilm productivity than its ancestor under any condition, while it was indeed reduced in mixed-species biofilms. Such observation aligned with the reduced abundance of TasA, a major biofilm matrix component in Bacillus species, and SpoVG, a regulator of sporulation, as revealed by matrix proteomics analysis. Furthermore, the variant showed shorter generation time and a lack of sporulation compared to its ancestor, consistent with mutations in key genes for regulating sporulation. Our results indicate that interspecies interactions within biofilms promote B. thuringiensis diversification and alter traits such as biofilm matrix production. Although sporulation is a survival mechanism, this study provides evidence that sporulation does not confer a fitness advantage in in vitro biofilm setting, even within mixed cultures, in the absence of severe stress.
HostingRepository	PRIDE
AnnounceDate	2025-06-19
AnnouncementXML	Submission_2025-06-19_07:43:40.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Amador
SpeciesList	scientific name: Pseudomonas brenneri; NCBI TaxID: 129817; scientific name: Bacillus thuringiensis; NCBI TaxID: 1428;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-16 03:35:32	ID requested
⏵ 1	2025-06-19 07:43:40	announced

10.1038/S41522-025-00755-1;

submitter keyword: Pseudomonas, Bacillus,bacteria, matrix, biofilms

Cristina Amador
contact affiliation	Section of Microbiology, Department of Biology University of Copenhagen
contact email	chierro@bio.ku
lab head
Cristina Amador
contact affiliation	Copenhagen University
contact email	chierro@bio.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046156
     1. Label: PRIDE project
     2. Name: Evolution of genotypic and phenotypic diversity in multispecies biofilms