PXD046133 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics study reveals that ASFV g5Rp protein interacts with eukaryotic translation initiation factor 5A and may regulate host translation |
| Description | African Swine fever virus (ASFV) decapacitase (g5Rp) hydrolyses the 5’ cap structure on cellular mRNA, facilitating the expression of viral genes in the early stages of ASFV infection. To investigate how g5Rp affects host translation would be beneficial for the prevention and control of ASFV infection and transmission. In this study, firstly we purified the g5Rp protein and investigated its protease activity, and secondly we performed a label-free quantitative proteomic assay to study the dynamic changes in the proteome of porcine alveolar macrophages (3D/421) overexpressing g5Rp. Analyzing 122 differentially expressed host cell proteins, we focused on the majority of ribosome-related upregulated protein expression in the protein family (eIF) belonging to the eukaryotic translation starter factor, notably downregulation of EIF2S1 and EIF5A. 7 proteins that may interact with g5Rp were further identified with IP-MS, and EIF5A and g5Rp were identified through CO-IP and PLA assays. Protein crystallization and structural analysis revealed that g5Rp, a dimer protein with Nudix domain, promotes ASFV replication by modulating translation in host cells. The present findings provide new insights into the mechanism of g5Rp involvement in host cell translation and lay the foundation for the prevention and control of ASFV. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-30 |
| AnnouncementXML | Submission_2025-11-30_02:30:10.898.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | chunmei xu |
| SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825; |
| ModificationList | (18)O label at both C-terminal oxygens |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-10-13 12:21:25 | ID requested | |
| ⏵ 1 | 2025-11-30 02:30:11 | announced | |
Publication List
Keyword List
| submitter keyword: crystal structure, EIF5A,African swine fever virus, protein-protein interaction, g5Rp, LC-MS/MS |
Contact List
| xiaomin Zhao |
|---|
| contact affiliation | College of Veterinary Medicine, Northwest A&F University, Yangling, Shaaxi 712100, P. R. |
| contact email | xiaominz@nwafu.cn |
| lab head | |
| chunmei xu |
|---|
| contact affiliation | Key Laboratory of Animal Biosafety Risk Prevention and Control (North) and Key Laboratory of Veterinary Biological Products and Chemical Drugs of Ministry of Agriculture and Rural Affairs (MARA), Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. |
| contact email | xcm13592563704@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046133
- Label: PRIDE project
- Name: Proteomics study reveals that ASFV g5Rp protein interacts with eukaryotic translation initiation factor 5A and may regulate host translation
