PXD046060-1

PXD046060 is an original dataset announced via ProteomeXchange.

Title	Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): Hints of cultivation stress-induced genomic changes
Description	Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents. Within this study we focused on the edible dormouse (Glis glis), a rodent with unique life-history traits that often enters households and whose possible role in the epidemiology of Bartonella infections had been previously unknown. We identified and cultivated two distinct Bartonella sub(species) significantly diverging from previously described species, which were characterized using growth characteristics, biochemical tests, and various molecular techniques including also proteomics. Two novel (sub)species were described: Bartonella grahamii subsp. shimonis subsp. nov. and Bartonella gliris sp. nov. We sequenced two individual strains per each described (sub)species. During exploratory genomic analyses comparing two genotypes ultimately belonging to the same species, both factually and most importantly even spatiotemporally, we noticed unexpectedly significant structural variation between them. We found that most of the detected structural variants could be explained either by prophage excision or integration. Based on a detailed study of one such event, we argue that prophage deletion represents the most probable explanation of the observed phenomena. Moreover, in one strain of Bartonella grahamii subsp. shimonis subsp. nov. we identified a deletion related to Bartonella Adhesin A, a major pathogenicity factor that modulates bacteria-host interactions. Altogether, our results suggest that even a limited number of passages induced sufficient selective pressure to promote significant changes at the level of the genome.
HostingRepository	PRIDE
AnnounceDate	2023-10-28
AnnouncementXML	Submission_2023-10-28_07:00:24.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JiriDRESLER
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Bartonella grahamii; NCBI TaxID: 33045;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-11 06:05:12	ID requested
⏵ 1	2023-10-28 07:00:24	announced
2	2023-11-14 08:26:31	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:26	announced	2024-10-22: Updated project metadata.

submitter keyword: Bartonella, proteomics, Q-Exactive, culture, adhesin, BadA protein

JiriDresler,
contact affiliation	Military Health Institute, Prague, Czech Republic
contact email	jiri.dresler@gmail.com
lab head
JiriDRESLER
contact affiliation	Military Health Institute, Prague/Czech Republic
contact email	jiri.dresler@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046060
     1. Label: PRIDE project
     2. Name: Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): Hints of cultivation stress-induced genomic changes