PXD046049-1

PXD046049 is an original dataset announced via ProteomeXchange.

Title	Matrix metalloproteinase landscape in the imiquimod-induced skin inflammation mouse model
Description	Inflammation and autoimmunity are known as central processes in many skin diseases, including psoriasis. It is therefore important to develop pre-clinical models that describe disease-related aspects to enable testing of pharmaceutical drug candidates and formulations to essentially develop safe and effective treatments. A widely accepted pre-clinical model of psoriasis is the imiquimod (IMQ)-induced skin inflammation mouse model. It is primarily used to assess safety and efficacy of drug candidates for treatment of psoriasis but can also be used to evaluate the performance of drug delivery systems. In this model, topically applied IMQ provokes local skin inflammation, which is characterized by elevated levels of cytokines and chemokines, cellular infiltration, and increased epidermal thickness. In this study, we investigated the abundance of a subset of matrix metalloproteinases (MMPs) in skin from mice with IMQ-induced skin inflammation and skin from naïve mice using targeted proteomics. Our findings revealed a significant increase in the abundance of MMP-2, MMP-7, MMP-8, and MMP-13 after treatment with IMQ compared to the control skin, while MMP-3, MMP-9, and MMP-10 were exclusively detected in the IMQ-treated skin. The increased abundance and broader representation of MMPs in the IMQ-treated skin provide valuable insight into the pathophysiology of skin inflammation in the IMQ model. Specifically, the changes in the MMP profiles observed in the IMQ-treated skin resemble the MMP patterns found in skin lesions of individuals with psoriasis. Ultimately, the differences in MMP abundance under IMQ-induced inflammation as compared to non-inflamed control skin can be exploited as a model to investigate drug efficacy or performance of drug delivery systems.
HostingRepository	PanoramaPublic
AnnounceDate	2025-10-31
AnnouncementXML	Submission_2025-10-31_15:53:23.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vahap Canbay
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-10 22:30:33	ID requested
⏵ 1	2025-10-31 15:53:24	announced

Noddeland HK, Canbay V, Lind M, Savickas S, Jensen LB, Petersson K, Malmsten M, Koch J, Auf dem Keller U, Heinz A, Matrix metalloproteinase landscape in the imiquimod-induced skin inflammation mouse model. Biochimie, 226():99-106(2024) [pubmed]

submitter keyword: Parallel reaction monitoring, pre-clinical animal model, proteases, psoriasis, skin inflammation, targeted proteomics

Konstantinos Kalogeropoulos
contact affiliation	Technical University of Denmark
contact email	uadk@dtu.dk
lab head
Vahap Canbay
contact affiliation	Technical University of Denmark
contact email	vahcan@dtu.dk
dataset submitter

Panorama Public dataset URI