PXD046028-1

PXD046028 is an original dataset announced via ProteomeXchange.

Title	Identification of biomarkers of central nervous system damage in mice of benzene poisoning based on TMT proteomics
Description	Benzene is known to be a common toxic industrial chemical, and prolonged benzene exposure may cause nervous system damage. At present, there were few studies on benzene-induced neurological damage. This research aimed to identify protein biomarkers to predict central nervous system damage of benzene poisoning. We established a benzene poisoning model of C57 mice by gavage of benzene-peanut oil suspension and identified differentially expressed proteins (DEPs) in brain tissue using TMT proteomics. The results showed a significant weight loss and decrease in leukocyte and neutrophil counts in benzene poisoning mice compared to the control group. We also observed significant cerebral oedema and enhanced basophilic of neurons in the hippocampus in benzene poisoning group of mice. TMT proteomic results showed that a total 6985 proteins were quantified, with a Fold Change (FC) > 1.2 (or <1/1.2) and P value <0.05 were considered as DEPs. Compared with the control group, we identified 43 DEPs, comprising 14 upregulated and 29 downregulated proteins. KEGG pathway analysis showed that the candidate proteins were mainly involved in cholesterol metabolism, complement and coagulation cascades, african trypanosomiasis, PPAR signaling pathway and vitamin digestion and absorption. Three proteins, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase (UGT8), Apolipoprotein A-I (APOA1) and Complement C3 (C3) were validated using immunoblotting and immunohistochemical. In conclusion, our results may provide some molecular biomarkers for identifying the nervous system damage of benzene poisoning.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_11:51:48.226.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Zheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-10 05:31:26	ID requested
⏵ 1	2025-05-06 11:51:49	announced

10.1093/toxres/tfae036;

Zheng Z, Li H, Zhang Z, Zhai X, Qin H, Study on the underlying molecular mechanism of benzene-induced nervous system damage in mice based on tandem mass tag (TMT) proteomics. Toxicol Res (Camb), 13(2):tfae036(2024) [pubmed]

submitter keyword: benzene poisoning

TMT proteomic

brain

mice

Zhe Zheng
contact affiliation	Department of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, 471023, China
contact email	forensiczheng@sina.com
lab head
Zhe Zheng
contact affiliation	Henan University of Science and Technology
contact email	forensiczheng@sina.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046028
     1. Label: PRIDE project
     2. Name: Identification of biomarkers of central nervous system damage in mice of benzene poisoning based on TMT proteomics