PXD046005-1

PXD046005 is an original dataset announced via ProteomeXchange.

Title	Effect of oxygen tension on MMTV-PyMT tumor cell kinome
Description	Solid tumors are characterized by significantly lower oxygen (O2) levels. Despite this fact, routine preclinical cancer studies are performed under ambient O2 conditions. We have developed an experimental approach that allows us to collect, process and evaluate cancer and non-cancer cells under physioxia (3-5% O2), in such a way that there is very minimal exposure to air. We have shown in previous studies that ambient and physioxic O2 differentially impact relevant cell surface markers and sensitivity to therapy. In this study, our goal was to further explore oxygen-dependent signaling pathway alterations and to determine how these pathways influence response to targeted therapies. Using cells derived from transgenic mouse mammary tumors and healthy primary human breast epithelial cells, we show the impact of ambient air and physioxia on the kinome, with subsequent pathway alterations that mediate the effectiveness of single and combination therapies. Our data emphasize the importance of O2 considerations in preclinical cancer research and drug development.
HostingRepository	PRIDE
AnnounceDate	2024-05-08
AnnouncementXML	Submission_2024-05-08_08:33:16.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Angus
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-09 15:31:37	ID requested
⏵ 1	2024-05-08 08:33:16	announced
2	2024-10-22 06:38:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ambient,breast cancer, kinase inhibitor beads, physioxia, lapatinib

Hari Nakshatri, PhD
contact affiliation	Indiana University School of Medicine
contact email	hnakshat@iupui.edu
lab head
Steven Angus
contact affiliation	Indiana University School of Medicine
contact email	sangus@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046005
     1. Label: PRIDE project
     2. Name: Effect of oxygen tension on MMTV-PyMT tumor cell kinome