PXD045961-1

PXD045961 is an original dataset announced via ProteomeXchange.

Title	Overexpress S-protein tagged mouse TBK1 protein and identify the TBK1-interacting proteins by mass spectrometry.
Description	HEK293T cells were transfected with SFB-tagged mTBK1 or an empty vector for 24 h, the cells were lysed with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, 1 mM ethylenediaminetetraacetic acid) containing a complete protease inhibitor cocktail, followed by centrifugation at 13000r for 10 min at 4 °C. The supernatants were subjected to immunoprecipitation using S-protein agarose (Millipore, Cat# 69704) for 3 h at 4 °C. Immunoprecipitates were washed with lysis buffer containing 150 mM NaCl three times and incubated with cell lysates from RAW 264.7 cells overnight at 4 °C. The immunoprecipitated complexes were washed three times again and separated by SDS-PAGE, and the gel was stained with Coomassie brilliant blue. The entire lane was cut into 2-mm gel slices, digested with Trypsin, and subjected to LC-MS/MS assays using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). The mass spectrometry data were analyzed using Thermo Proteome Discovery (version2.3), and tandem mass spectra were searched against the UniProt-Homo sapiens database.
HostingRepository	iProX
AnnounceDate	2023-10-07
AnnouncementXML	Submission_2023-10-07_20:16:39.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-07 20:16:21	ID requested
⏵ 1	2023-10-07 20:16:39	announced
2	2023-12-04 20:58:48	announced	2023-12-05: Update publication information.

Dataset with its publication pending

submitter keyword: SFB-tagged mTBK1, RAW 264.7 cells, LC-MS/MS assays

Qinmiao Sun
contact affiliation	Institute of Zoology, Chinese Academy of Sciences
contact email	qinmiaosun@ioz.ac.cn
lab head
Jing Yang
contact affiliation	Institute of Zoology, Chinese Academy of Sciences
contact email	yangjing22@ioz.ac.cn
dataset submitter

iProX dataset URI