PXD045948-1

PXD045948 is an original dataset announced via ProteomeXchange.

Title	FGL1 IP-MS data in 293T cell lysates
Description	We immunoprecipitated exogenously expressed Myc-FGL1 using Anti-Myc-Tag monoclonal antibody (DIA-AN, Wuhan, China, Cat#2097) from 293T cells cocultured with tumor associated macrophages (TAMs) for 16 hr. No FGL1 protein was identified in protein lysates in which normal mouse IgG (Santa Cruz, California, USA, Cat#sc-2025) was added. Thus, protein lysates of 293T cells cocultured with TAMs added to mouse IgG were utilized as the negative control. A high-throughput technology, LC/MS (MS), was applied to identify the candidate interacting proteins of FGL1.
HostingRepository	iProX
AnnounceDate	2023-10-03
AnnouncementXML	Submission_2023-10-06_23:40:01.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiajia Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-06 23:39:38	ID requested
⏵ 1	2023-10-06 23:40:01	announced
2	2023-12-04 21:00:00	announced	2023-12-05: Update publication information.

Dataset with its publication pending

submitter keyword: FGL1, Immunoprecipitation, Mass spectrometry, Coculture with tumor-associated macrophages

Huaiqiang Ju
contact affiliation	Sun Yat-sen University Cancer Center
contact email	juhq@sysucc.org.cn
lab head
Jiajia Li
contact affiliation	Sun Yat-sen University Cancer Center
contact email	lijj2@sysucc.org.cn
dataset submitter

iProX dataset URI