PXD045926-1

PXD045926 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of Saliva from Chewing Tobacco Users and Oral Cancer Patients Reveals Shared Biomarkers
Description	Objectives: The aim of this study was to investigate the salivary proteomic profile of smokeless tobacco users in comparison to non-smokers using LC-MS/MS and to investigate the biological changes associated with smokeless tobacco use and oral cancer. Materials & Methods: Saliva samples of 65 participants were collected and divided into three groups: control (25 participants) , smokeless tobacco users group (25 participant), and oral cancer group (15 participant). The mean ages of the control participants, smokeless tobacco users, and oral cancer patients were 37.08 years, 37.8 years, and 52 years, respectively. The saliva samples were then prepared for LC-MS/MS analysis through in-solution digestion. The raw data files were processed using MaxQuant 2.1.0.0 with the human canonical proteome database. Results: The analysis revealed that 343 protein groups exhibited significantly altered abundance in the saliva samples. Among these, 43 out of 51 dysregulated proteins in the smokeless tobacco group were also dysregulated in the oral cancer group. Notably, Apolipoprotein A1 (ApoA1) and Pon1 were found to be significantly increased in both smokeless tobacco users and oral cancer patients. Furthermore, six out of the 20 most significantly altered proteins were mitochondrial proteins, and all of them were decreased relative to controls in both smokeless tobacco users and cancer samples.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-17_06:34:14.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Giddey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-06 04:25:16	ID requested
⏵ 1	2025-11-17 06:34:15	announced

10.1016/J.JDENT.2024.105268;

submitter keyword: Saliva, Oral cancer, Proteomics,Chewing Tobacco, LC-MS/MS

Mohammed H Semreen
contact affiliation	Sharjah Institute for Medical Research & Health Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates
contact email	msemreen@sharjah.ac.ae
lab head
Alexander Giddey
contact affiliation	University of Cape Town
contact email	gddale001@myuct.ac.za
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD045926

PRIDE project URI

• PRIDE
  1. PXD045926
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of Saliva from Chewing Tobacco Users and Oral Cancer Patients Reveals Shared Biomarkers