PXD045914-2

PXD045914 is an original dataset announced via ProteomeXchange.

Title	The MCM helicase displays distinct roles controlling dNTP synthesis and preventing genetic instability through physical interactions with the RNR complex
Description	The helicase MCM and the ribonucleotide reductase RNR are the complexes that provide the substrates (ssDNA templates and dNTPs, respectively) for DNA replication. Here, we demonstrate that MCM interacts physically with RNR and some of its regulators, including the kinases Rad53 and Dun1. Partial disruption of the MCM/RNR interactions increases the levels of dNTPs under unperturbed conditions, pointing to a role for MCM counteracting excessive RNR activity. In response to DNA damage, most of these physical interactions augment and this increase depends on Dun1. Partial disruption of the MCM/RNR interactions does not affect the pool of dNTPs under these conditions. However, this disruption impairs the release of Rad52 from the DNA repair centers despite the lesions are repaired, leading to hypermutagenesis and sensitivity to genotoxic agents. Therefore, the MCM/RNR interactions play non-canonical roles preventing by distinct mechanisms excessive dNTP synthesis and persistent Rad52 centers.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:39:19.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Román González-Prieto
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-05 08:17:30	ID requested
1	2024-05-15 01:47:25	announced
⏵ 2	2024-10-22 06:39:20	announced	2024-10-22: Updated project metadata.

10.1371/JOURNAL.PGEN.1011148;

submitter keyword: MCM/ RNR/ Rad52/ RPA/ dNTP/ mutagenesis/ recombination

Román González Prieto
contact affiliation	CABIMER-University of Sevilla
contact email	roman.gonzalez@cabimer.es
lab head
Román González-Prieto
contact affiliation	Andalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact email	roman.gonzalez@cabimer.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045914
     1. Label: PRIDE project
     2. Name: The MCM helicase displays distinct roles controlling dNTP synthesis and preventing genetic instability through physical interactions with the RNR complex