PXD045905-1

PXD045905 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of Purified Calpain-2 and Secondary Lipid Oxidation Products In-Vitro with LC-MS/MS and MALDI
Description	Secondary lipid oxidation products modify and impact the function of proteins. Identifying where and how these products modify a protein's structure would allow for targeted approaches to mitigate or prevent this modification. Here, we purified calpain-2, a neutral calcium dependent cysteine protease, from porcine skeletal muscle and conducted in-vitro experiments using various concentrations of malondialdehyde, 2-hexenal, and 4-hydroxynonenal. We determined these secondary lipid oxidation products differentially impact calpain-2 proteolytic activity and autolysis. We identified modifications of malondialdehyde and hexenal on calpain-2 with LC-MS/MS approaches. We further evaluated the number of adduction sites on the catalytic and regulatory subunits of calpain-2 through MALDI-MS approaches. These data demonstrate that secondary lipid oxidation products can modify and differentially impact calpain-2.
HostingRepository	MassIVE
AnnounceDate	2024-05-14
AnnouncementXML	Submission_2024-05-14_11:05:42.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Logan Johnson
SpeciesList	scientific name: Sus scrofa; common name: pig; NCBI TaxID: 9823;
ModificationList	Oxidation; S-carboxamidomethyl-L-cysteine; malondialdehyde lysine adduct (+54 amu); HNE; unknown modification
Instrument	Orbitrap Eclipse; ultrafleXtreme

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-04 15:10:45	ID requested
⏵ 1	2024-05-14 11:05:42	announced

Publication List

Logan G. Johnson, Chaoyu Zhai, Kitty Brown, Jessica E. Prenni, Mahesh N. Nair., Elisabeth Huff-Lonergan, and Steven M. Lonergan. Secondary Lipid Oxidation Products as Modulators of Calpain-2 Functionality In Vitro. J. Agric. Food Chem. 2024. doi: 10.1021/acs.jafc.4c00335.

Logan G. Johnson, Chaoyu Zhai, Kitty Brown, Jessica E. Prenni, Mahesh N. Nair., Elisabeth Huff-Lonergan, and Steven M. Lonergan. Secondary Lipid Oxidation Products as Modulators of Calpain-2 Functionality In Vitro. J. Agric. Food Chem. 2024. doi: 10.1021/acs.jafc.4c00335.

Keyword List

submitter keyword: in-vitro, malondialdehyde, hexenal, 4-hydroxynonenal, calpain-2

submitter keyword: in-vitro, malondialdehyde, hexenal, 4-hydroxynonenal, calpain-2

Contact List

Steven Lonergan
contact affiliation	Iowa State University
contact email	slonerga@iastate.edu
lab head
Logan Johnson
contact affiliation	Iowa State University
contact email	logan01@iastate.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093040/