PXD045869-1
PXD045869 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA) |
| Description | Aspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig binding was different between asthmatic and healthy horses’ sera. Proteins were in-gel digested and tryptic peptides extracted from the 21 spots of interest, analyzed by liquid chromatography-mass spectrometry and eight identified and prioritized candidate antigens were expressed as recombinant proteins. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2024-02-13 |
| AnnouncementXML | Submission_2024-02-13_17:12:55.538.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Daniela Volke |
| SpeciesList | scientific name: Aspergillus fumigatus; NCBI TaxID: 746128; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | SYNAPT G2-Si |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-02 20:46:07 | ID requested | |
| ⏵ 1 | 2024-02-13 17:12:55 | announced | |
| 2 | 2024-04-29 14:25:01 | announced | : Added PubMed Id |
Publication List
| Jentsch M-C, Lübke S, Schrödl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B and Schnabel CL (2024) Immunoproteomics enables broad identification of new Aspergillus fumigatus antigens in severe equine asthma. Front. Immunol. 15:1347164. doi: 10.3389/fimmu.2024.1347164 |
Keyword List
| submitter keyword: recurrent airway obstruction, COPD, proteomics, immunoglobulin isotype, serum, antibody, immunoblot |
Contact List
| Ralf Hoffmann | |
|---|---|
| contact affiliation | Universität Leipzig/BBZ |
| contact email | ralf.hoffmann@bbz.uni-leipzig.de |
| lab head | |
| Daniela Volke | |
| contact affiliation | Universität Leipzig/BBZ |
| contact email | daniela.volke@bbz.uni-leipzig.de |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
to receive all new ProteomeXchange dataset release announcements!
