PXD045869

PXD045869 is an original dataset announced via ProteomeXchange.

Title	In-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA)
Description	Aspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig binding was different between asthmatic and healthy horses’ sera. Proteins were in-gel digested and tryptic peptides extracted from the 21 spots of interest, analyzed by liquid chromatography-mass spectrometry and eight identified and prioritized candidate antigens were expressed as recombinant proteins.
HostingRepository	PanoramaPublic
AnnounceDate	2024-04-29
AnnouncementXML	Submission_2024-04-29_14:25:00.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniela Volke
SpeciesList	scientific name: Aspergillus fumigatus; NCBI TaxID: 746128;
ModificationList	Carbamidomethyl; Oxidation
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 20:46:07	ID requested
1	2024-02-13 17:12:55	announced
⏵ 2	2024-04-29 14:25:01	announced	: Added PubMed Id

Jentsch MC, L, ü, bke S, Schr, ö, dl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B, Schnabel CL, antigens in severe equine asthma. Front Immunol, 15():1347164(2024) [pubmed]

submitter keyword: recurrent airway obstruction, COPD, proteomics, immunoglobulin isotype, serum, antibody, immunoblot

Ralf Hoffmann
contact affiliation	Universität Leipzig/BBZ
contact email	ralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliation	Universität Leipzig/BBZ
contact email	daniela.volke@bbz.uni-leipzig.de
dataset submitter

Panorama Public dataset URI