<<< Full experiment listing


PXD045869 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn-Gel Digest to identify Aspergillus fumigatus protein antigens in severe equine asthma (SEA)
DescriptionAspergillus fumigatus (Asp f) has been described as a major extrinsic stimulus of SEA and is a common mold species in hay. Aiming to identify disease-relevant antigens, we analyzed Asp f as a source, in an immuno-proteomics approach on two-dimensional immunoblots. For 21 of 289 spots serum Ig binding was different between asthmatic and healthy horses’ sera. Proteins were in-gel digested and tryptic peptides extracted from the 21 spots of interest, analyzed by liquid chromatography-mass spectrometry and eight identified and prioritized candidate antigens were expressed as recombinant proteins.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDaniela Volke
SpeciesList scientific name: Aspergillus fumigatus; NCBI TaxID: 746128;
ModificationListCarbamidomethyl; Oxidation
InstrumentSYNAPT G2-Si
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-02 20:46:07ID requested
12024-02-13 17:12:55announced
22024-04-29 14:25:01announced: Added PubMed Id
Publication List
Jentsch MC, L, ü, bke S, Schr, ö, dl W, Volke D, Krizsan A, Hoffmann R, Kaiser-Thom S, Gerber V, Marti E, Wagner B, Schnabel CL, antigens in severe equine asthma. Front Immunol, 15():1347164(2024) [pubmed]
Keyword List
submitter keyword: recurrent airway obstruction, COPD, proteomics, immunoglobulin isotype, serum, antibody, immunoblot
Contact List
Ralf Hoffmann
contact affiliationUniversität Leipzig/BBZ
contact emailralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliationUniversität Leipzig/BBZ
contact emaildaniela.volke@bbz.uni-leipzig.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI