PXD045866 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Etomoxir as a Promiscuous Chemoproteomic Probe. |
Description | Primary hepatocytes from WT or Pex5KO mouse livers were treated with 100uM clickable etomoxir analogues and the proteins bound to etomoxir were pulled down and identified. The small molecule inhibitor etomoxir has been used for many years as a specific inhibitor of Cpt1 but these data show that etomoxir binds a large array of proteins and is not appropriate as a tool for evaluating the biological effects of fatty acid oxidation. Primary hepatocytes were treated with 100uM clickable etomoxir analogues and the proteins bound to etomoxir were pulled down and identified. The small molecule inhibitor etomoxir has been used for many years as a specific inhibitor of Cpt1 but these data show that etomoxir binds a large array of proteins and is not appropriate as a tool for evaluating the biological effects of fatty acid oxidation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_03:57:59.427.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Wolfgang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-02 13:43:45 | ID requested | |
⏵ 1 | 2024-10-17 03:58:00 | announced | |
Publication List
Choi J, Smith DM, Lee YJ, Cai D, Hossain MJ, O'Connor TJ, Deme P, Haughey NJ, Scafidi S, Riddle RC, Wolfgang MJ, Etomoxir repurposed as a promiscuous fatty acid mimetic chemoproteomic probe. iScience, 27(9):110642(2024) [pubmed] |
10.1016/j.isci.2024.110642; |
Keyword List
submitter keyword: Liver, Fatty Acid Oxidation, Cpt1, Etomoxir |
Contact List
Michael J. Wolfgang |
contact affiliation | The Johns Hopkins University School of Medicine, Baltimore, Maryland, Department of Biological Chemistry and Department of Pharmacology and Molecular Sciences |
contact email | mwolfga1@jhmi.edu |
lab head | |
Michael Wolfgang |
contact affiliation | Johns Hopkins University School of Medicine |
contact email | mwolfga1@jhmi.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD045866 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045866
- Label: PRIDE project
- Name: Etomoxir as a Promiscuous Chemoproteomic Probe.