PXD045849 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Isolation and characterization of extracellular vesicles from Arabidopsis thaliana cell culture and investigation of the specificities of their biogenesis |
| Description | Over the past decade, membrane vesicles, known as exosomes, have garnered significant attention due to their potential as natural nanocarriers. Recently, it was discovered that plants also release extracellular vesicles (EVs). Yet, our understanding of EV production in plant cell culture remains limited. In this study, we used callus culture of the model plant Arabidopsis thaliana to isolate and comprehensively characterize EVs emanating from cultivated cells. Our findings reveal that the quantity of EVs in calli was significantly lower than in the plant's apoplastic fluid. This discrepancy is linked to the transcriptional repression of the endosomal sorting complex required for transport (ESCRT) genes. While salicylic acid elevated the expression of ESCRT components, it did not boost EV production. Remarkably, EVs from calli encompass proteins pivotal for cell wall biogenesis and defense mechanisms, as well as microRNAs consistent with those in intact plants. This indicates that plant cell cultures might be a viable source of EVs reflecting the properties of the parent plant species. Nevertheless, additional research is crucial to pinpoint the optimal conditions for efficient EV production. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-01-26 |
| AnnouncementXML | Submission_2024-01-26_07:37:53.946.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD045849 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Dmitriy Bulgakov |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | autoflex |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-10-02 06:39:47 | ID requested | |
| ⏵ 1 | 2024-01-26 07:37:54 | announced | |
Publication List
| 10.3390/plants12203604; |
| Yugay Y, Tsydeneshieva Z, Rusapetova T, Grischenko O, Mironova A, Bulgakov D, Silant'ev V, Tchernoded G, Bulgakov V, Shkryl Y, Cell Culture and Investigation of the Specificities of Their Biogenesis. Plants (Basel), 12(20):(2023) [pubmed] |
| 10.6019/PXD045849; |
Keyword List
| submitter keyword: exosomes |
| nanoparticles |
| membrane trafficking |
| callus culture |
| salicylic acid |
Contact List
| Yuri Nikolaevich Shkryl |
|---|
| contact affiliation | Head of the Laboratory of bionanotechnology and biomedicine, Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia |
| contact email | yn80@mail.ru |
| lab head | |
| Dmitriy Bulgakov |
|---|
| contact affiliation | Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok-22, 690022, Russia |
| contact email | bulgakov-dv@mail.ru |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045849 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045849
- Label: PRIDE project
- Name: Isolation and characterization of extracellular vesicles from Arabidopsis thaliana cell culture and investigation of the specificities of their biogenesis
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