PXD045848-1

PXD045848 is an original dataset announced via ProteomeXchange.

Title	Analysis of RNA-dependent proteins in the cyanobacterium Synechocystis by RNase sensitive gradient profiling (Grad-R) Analysis of RNA-dependent proteins in the cyanobacterium Synechocystis by RNase sensitive gradient profiling (Grad-R)
Description	RNA-binding proteins (RBPs) are an essential component of ribonucleoprotein complexes and are crucial for post-transcriptional regulation by regulatory sRNAs. Well-studied examples in different groups of bacteria include RBPs such as various ribosomal and RRM-domain containing proteins, or the RNA chaperones Hfq, ProQ and CsrA. However, the knowledge about RBPs and possible RNA chaperones in photosynthetic cyanobacteria is very limited, although there is extensive evidence for the presence of post-transcriptional regulation. Here, we analyzed cellular protein complexes by gradient profiling with or without RNase treatment (Grad-R) in Synechocystis sp. PCC 6803.
HostingRepository	PRIDE
AnnounceDate	2025-08-26
AnnouncementXML	Submission_2025-08-26_01:55:27.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-02 06:26:09	ID requested
⏵ 1	2025-08-26 01:55:27	announced

Dataset with its publication pending

submitter keyword: cyanobacteria / post-transcriptional regulation / ribonucleoprotein complexes / RNA-binding proteins / small regulatory RNAs / sucrose-gradient fractionation

Dörte Becher
contact affiliation	Department of Microbial Proteomics, Institute of Microbiology, Center for Functional Genomics of Microbes, University of Greifswald, D-17489 Greifswald, Germany.
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045848
     1. Label: PRIDE project
     2. Name: Analysis of RNA-dependent proteins in the cyanobacterium Synechocystis by RNase sensitive gradient profiling (Grad-R) Analysis of RNA-dependent proteins in the cyanobacterium Synechocystis by RNase sensitive gradient profiling (Grad-R)