PXD045835 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pseudomonas aeruginosa Heme Metabolites Biliverdin IX and IX are Integral to Lifestyle Adaptations Associated with Chronic Infection |
Description | Pseudomonas aeruginosa is a versatile opportunistic pathogen requiring iron for its survival and virulence within the host. The ability to switch to heme as an iron source provides an advantage in chronic infection. We have recently shown the extracellular heme metabolites biliverdin IX (BVIX) and/or BVIX positively regulate the heme dependent cell surface signaling cascade. We further investigated the role of BVIX and BVIX in cell signaling utilizing allelic strains lacking a functional HemO (hemOin), or one reengineered to produce BVIX (hemO). Compared to PAO1 both strains show a heme dependent growth defect, decreased swarming and twitching and less robust biofilm formation. Interestingly, the motility and biofilm defects were partially rescued on addition of exogenous BVIX and BVIX. Utilizing LC-MS/MS we performed a comparative proteomics and metabolomics analysis of PAO1 versus the allelic strains in shaking and static conditions. In shaking conditions, the hemO allelic strains showed a significant increase in proteins involved in quorum sensing (QS), phenazine production and chemotaxis.Metabolite profiling further revealed increased levels of PQS and phenazine metabolites. In static conditions we observed a significant repression of chemosensory pathways and Type IV pili (TFP) biogenesis proteins as well as several phosphodiesterases associated with biofilm dispersal. We propose BVIX metabolites function as signaling and chemotactic molecules integrating heme utilization as an iron source into the adaptation of P. aeruginosa from a planktonic to sessile lifestyle. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-19 |
AnnouncementXML | Submission_2024-01-19_13:27:05.641.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mehari Weldemariam |
SpeciesList | scientific name: Pseudomonas aeruginosa PABL056; NCBI TaxID: 1214916; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-29 18:25:26 | ID requested | |
⏵ 1 | 2024-01-19 13:27:06 | announced | |
Publication List
Keyword List
submitter keyword: proteomics, metabolomics, Type IV pili and chemotaxis, heme, biliverdin,Pseudomonas aeruginosa |
Contact List
Kane Maureen |
contact affiliation | Department of Pharmaceutical Sciences School of Pharmacy University of Maryland Baltimore |
contact email | mkane@rx.umaryland.edu |
lab head | |
Mehari Weldemariam |
contact affiliation | University of Maryland Baltimore |
contact email | mweldemariam@rx.umaryland.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045835
- Label: PRIDE project
- Name: Pseudomonas aeruginosa Heme Metabolites Biliverdin IX and IX are Integral to Lifestyle Adaptations Associated with Chronic Infection