PXD045827-1
PXD045827 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An inhibitor/anti-inhibitor system controls the activity of lytic transglycosylase MltF in Pseudomonas aeruginosa |
| Description | Most bacterial cell envelopes contain a cell wall layer made of peptidoglycan. The synthesis of new peptidoglycan is critical for cell growth, division and morphogenesis, and is also coordinated with peptidoglycan hydrolysis to accommodate the new material. However, the enzymes that cleave peptidoglycan must be carefully controlled to avoid autolysis. In recent years, some control mechanisms have begun to emerge, although there are many more questions than answers for how most cell wall hydrolases are regulated. Here, we report a novel cell wall hydrolase control mechanism in Pseudomonas aeruginosa, which we discovered during our characterization of a mutant sensitive to the overproduction of a secretin protein. The mutation affected an uncharacterized Sel1-like repeat protein encoded by the PA3978 locus. In addition to the secretin-sensitivity phenotype, PA3978 disruption also increased resistance to a b-lactam antibiotic used in the clinic. Affinity purification followed by mass spectrometry on the C-terminal flag tagged PA3978 protein revealed that PA3978 binds to the lytic transglycosylase MltF. In vitro and In vivo analysis revealed that the binding occurs on the catalytic domain of the lytic transglycosylase MltF and inhibits its activity. We also discovered another interaction partner of PA3978 encoded by the PA5502 locus. The phenotypes of a deltaPA5502 mutant suggested that PA5502 interferes with the inhibitory function of PA3978 towards MltF, and we confirmed that activity for PA5502 in vitro. Therefore, PA3978 and PA5502 form an inhibitor/anti-inhibitor system that controls MltF activity. We propose to name these proteins IltA (inhibitor of lytic transglycosylase) and LiiA (lytic transglycosylase inhibitor, inhibitor). The mass spectrometry raw files and search results for the affinity purification of PA3978 are deposited here. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-10-09 |
| AnnouncementXML | Submission_2023-10-09_09:55:18.823.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Beatrix Ueberheide |
| SpeciesList | scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-29 13:06:42 | ID requested | |
| ⏵ 1 | 2023-10-09 09:55:19 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Pseudomonas aeruginosa, affinity purification mass spectrometry, lytic transglycosylase MltF |
Contact List
| Beatrix Ueberheide | |
|---|---|
| contact affiliation | NYU Langone Health |
| contact email | beatrix.ueberheide@nyulangone.org |
| lab head | |
| Beatrix Ueberheide | |
| contact affiliation | NYU School of Medicine |
| contact email | beatrixueberheide@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093002/ |
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