PXD045824-2

PXD045824 is an original dataset announced via ProteomeXchange.

Title	Dynamic metabolic efficiency of substrate utilization by copiotrophic marine bacteria
Description	Under well-defined laboratory conditions, we grew R. pomeroyi DSS-3 and A. macleodii MIT1002 in batch cultures on a monosaccharide (glucose) and organic acid (acetate), provided either individually or in combination, and all at the same carbon equivalent. This batch culturing approach mimicked bacterial DOC assimilation in short-lived substrate ‘hot spots’, such as those formed by high phytoplankton extracellular release at peak photon availability. Measurements were made of bacterial metabolite uptake, respiration, and biomass accumulation through a growth cycle. Insights into bacterial core metabolism came from gene and protein expression measured at intervals during growth. Curated genome-scale models (flux balance analysis; FBA) were used to explore the metabolic foundation of CO2 production for insights into determinants of BGE and bCUE.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:26.873.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045824
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew McIlvin
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Alteromonas macleodii; NCBI TaxID: 28108; scientific name: Ruegeria pomeroyi DSS-3; NCBI TaxID: 246200;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 11:15:25	ID requested
1	2023-10-27 11:20:42	announced
⏵ 2	2024-10-22 06:13:27	announced	2024-10-22: Updated project metadata.

10.6019/PXD045824;

submitter keyword: Alteromonas macleodii MIT1002, LCMS,Ruegeria pomeroyi DSS-3

Mak Saito
contact affiliation	Woods Hole Oceanographic Institution USA
contact email	msaito@whoi.edu
lab head
Matthew McIlvin
contact affiliation	Woods Hole Oceanographic Inst.
contact email	mmcilvin@whoi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045824
     1. Label: PRIDE project
     2. Name: Dynamic metabolic efficiency of substrate utilization by copiotrophic marine bacteria