PXD045820-1

PXD045820 is an original dataset announced via ProteomeXchange.

Title	Thermal proteome profilling in WT and CSE-/- MEF cells upon H2O2 treatment
Description	Cellular homeostasis relies on precise regulation through chemical processes, such as protein posttranslational modifications and physical processes, such as biomolecular condensation. Aging disrupts this balance, increasing susceptibility to diseases and death. However, the mechanisms behind age-related pathogenesis remain unclear. Using chemoproteomic methods to dissect different cysteine posttranslational modifications, we found that age-related changes in cysteine redox homeostasis impact proteins ability to form biomolecular condensates. Age-related increase in sulfenylation and sulfonylation promotes phase separation and through conformational change increases proteins propensity to aggregate. Causing condensates dissolution, protein persulfidation, a protective thiol modification, prevents this. Reduced persulfidation and increased sulfonylation in aging drive abnormal phase separation, leading to shorter life and spontaneous protein aggregation in CSE-/- mice. In this study, we assessed the stability of proteins in WT and CSE-/- Mouse embryonic fibroblast mice upon H2O2 treatment.
HostingRepository	PRIDE
AnnounceDate	2026-05-23
AnnouncementXML	Submission_2026-05-23_08:37:00.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Vignane
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 09:40:54	ID requested
⏵ 1	2026-05-23 08:37:01	announced

Dataset with its publication pending

submitter keyword: None

Milos Filipovic
contact affiliation	SULFGAGING group, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Germany
contact email	milos.filipovic@isas.de
lab head
Thibaut Vignane
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS,e.V, Dortmund, Germany
contact email	thibaut.vignane@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD045820

PRIDE project URI

• PRIDE
  1. PXD045820
     1. Label: PRIDE project
     2. Name: Thermal proteome profilling in WT and CSE-/- MEF cells upon H2O2 treatment