PXD045815-1
PXD045815 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis of mpk1/2/7/14 treated with mannitol or abscisic acid |
Description | Abscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG-binding domain 10 (MBD10) as a phosphoprotein that becomes differentially phosphorylated after ABA treatment in Arabidopsis. ABA-induced leaf senescence was delayed in mbd10 knockout plants but accelerated in MBD10-overexpressing plants, suggesting that MBD10 positively regulates ABA-induced leaf senescence. ABA-induced phosphorylation of MBD10 occurs in planta on Thr-89, and our results demonstrated that Thr-89 phosphorylation is essential for MBD10’s function in leaf senescence. The in vivo phosphorylation of Thr-89 in MBD10 was significantly downregulated in a quadruple mutant of group C MAPKs (mpk1/2/7/14), and group C MAPKs directly phosphorylated MBD10 in vitro. |
HostingRepository | jPOST |
AnnounceDate | 2023-09-30 |
AnnouncementXML | Submission_2023-09-29_08:10:05.607.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Taishi Umezawa |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-09-29 08:10:02 | ID requested | |
⏵ 1 | 2023-09-29 08:10:06 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Arabidopsis thaliana, LFQ |
Contact List
Taishi Umezawa | |
---|---|
lab head | |
Taishi Umezawa | |
contact affiliation | Tokyo University of Agriculture and Technology |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
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