PXD045806-1

PXD045806 is an original dataset announced via ProteomeXchange.

Title	Persulfidation and direct detection of sulfonylation in aging Brain of WT, CSE-/- and MPST-/- mice
Description	Cellular homeostasis relies on precise regulation through chemical processes, such as protein posttranslational modifications and physical processes, such as biomolecular condensation. Aging disrupts this balance, increasing susceptibility to diseases and death. However, the mechanisms behind age-related pathogenesis remain unclear. Using chemoproteomic methods to dissect different cysteine posttranslational modifications, we found that age-related changes in cysteine redox homeostasis impact proteins ability to form biomolecular condensates. Age-related increase in sulfenylation and sulfonylation promotes phase separation and through conformational change increases proteins propensity to aggregate. Causing condensates dissolution, protein persulfidation, a protective thiol modification, prevents this. Reduced persulfidation and increased sulfonylation in aging drive abnormal phase separation, leading to shorter life and spontaneous protein aggregation in CSE-/- mice. In this study, we assessed the changes in protein persulfidation levels in brain aging. For this, we used the frontal cortex of 10 weeks, 10 months and 18 months-old WT mice . To unravel the protective role of H2S against ROS, we used the thiol-blocked samples from the persulfidation protocol to directly detect cysteine sulfonylation by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2026-05-23
AnnouncementXML	Submission_2026-05-23_13:40:44.328.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD045806
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thibaut Vignane
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid)
Instrument	Orbitrap Eclipse; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 04:07:27	ID requested
⏵ 1	2026-05-23 13:40:44	announced

10.6019/PXD045806;

submitter keyword: None

Milos Filipovic
contact affiliation	SULFAGIN group, Leibniz-Institut für Analytische Wissenschaften, ISAS .eV, Germany
contact email	milos.filipovic@isas.de
lab head
Thibaut Vignane
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS,e.V, Dortmund, Germany
contact email	thibaut.vignane@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045806
     1. Label: PRIDE project
     2. Name: Persulfidation and direct detection of sulfonylation in aging Brain of WT, CSE-/- and MPST-/- mice