PXD045796 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence |
Description | Here, we describe a secreted HLA (sHLA) Fc-fusion construct for simple single HLA allele profiling in hypoxic pancreatic ductal adenocarcinoma (PDAC) and cellular senescence. This method streamlines sample preparation, enables temporal control, and provides allele-restricted target identification. Over 30,000 unique HLA-associated peptides were identified across two different HLA alleles and seven cell lines, with ~9,300 peptides newly discovered. The sHLA Fc-fusion capture technology holds potential to expedite immunopeptidomics and advance therapeutic interest in peptide-HLA complexes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_13:54:40.579.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lisa Kirkemo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-28 21:07:47 | ID requested | |
⏵ 1 | 2024-01-26 13:54:41 | announced | |
Publication List
10.1093/pnasnexus/pgad400; |
Rettko NJ, Kirkemo LL, Wells JA, Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence. PNAS Nexus, 2(12):pgad400(2023) [pubmed] |
Keyword List
submitter keyword: Immunopeptidomics, Senescence, Secreted HLA, Hypoxia |
Contact List
Jim Wells |
contact affiliation | Department of Pharmaceutical Chemistry School of Pharmacy UC San Francisco |
contact email | jim.wells@ucsf.edu |
lab head | |
Lisa Kirkemo |
contact affiliation | UC San Francisco |
contact email | lkirkemo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD045796 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045796
- Label: PRIDE project
- Name: Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence