PXD045791 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bioorthogonal Photocatalytic Proximity Labeling in Primary Living Samples |
Description | In situ profiling of subcellular proteomic networks in primary and living systems, such as primary cells from native tissues or clinic samples, is crucial for the understanding of life processes and diseases, yet challenging for the current proximity labeling methods (e.g., BioID, APEX) due to their necessity of genetic engineering. Here we report CAT-S, a state-of-the-art bioorthogonal photocatalytic chemistry-enabled proximity labeling method, that expands proximity labeling to a wide range of primary living samples for in situ profiling of subcellular proteomes. Powered by the newly introduced thioQM labeling warhead and targeted bioorthogonal photocatalytic decaging chemistry, CAT-S enables labeling of mitochondrial proteins in living cells with high efficiency and specificity (up to 87%). We applied CAT-S to diverse cell cultures, mouse tissues as well as primary T cells from human blood, portraying the native-state mitochondrial proteomic characteristics, and unveiled a set of hidden mitochondrial proteins in human proteome. Furthermore, CAT-S allows quantitative analysis of the in situ proteomic perturbations on dysfunctional tissue samples, exampled by diabetic mouse kidneys, and revealed the alterations of lipid metabolism machinery that drive the disease progression. Given the advantages of non-genetic operation, generality, efficiency as well as spatiotemporal resolution, CAT-S may open new avenues as a proximity labeling strategy for in situ investigation of subcellular proteomic landscape of primary living samples that are otherwise inaccessible. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_01:23:51.478.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ziqi Liu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-28 13:50:44 | ID requested | |
⏵ 1 | 2024-05-24 01:23:52 | announced | |
2 | 2024-10-22 06:42:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-024-46985-3; |
Liu Z, Guo F, Zhu Y, Qin S, Hou Y, Guo H, Lin F, Chen PR, Fan X, Bioorthogonal photocatalytic proximity labeling in primary living samples. Nat Commun, 15(1):2712(2024) [pubmed] |
Keyword List
submitter keyword: proximity labeling, mitochondria, bioorthogonal chemistry,photocatalysis |
Contact List
Xinyuan Fan |
contact affiliation | Peking University |
contact email | 1906885003@pku.edu.cn |
lab head | |
Ziqi Liu |
contact affiliation | College of Chemistry and Chemical Engineering, Peking University |
contact email | zqliu7@pku.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045791 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045791
- Label: PRIDE project
- Name: Bioorthogonal Photocatalytic Proximity Labeling in Primary Living Samples