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PXD045791-1

PXD045791 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBioorthogonal Photocatalytic Proximity Labeling in Primary Living Samples
DescriptionIn situ profiling of subcellular proteomic networks in primary and living systems, such as primary cells from native tissues or clinic samples, is crucial for the understanding of life processes and diseases, yet challenging for the current proximity labeling methods (e.g., BioID, APEX) due to their necessity of genetic engineering. Here we report CAT-S, a state-of-the-art bioorthogonal photocatalytic chemistry-enabled proximity labeling method, that expands proximity labeling to a wide range of primary living samples for in situ profiling of subcellular proteomes. Powered by the newly introduced thioQM labeling warhead and targeted bioorthogonal photocatalytic decaging chemistry, CAT-S enables labeling of mitochondrial proteins in living cells with high efficiency and specificity (up to 87%). We applied CAT-S to diverse cell cultures, mouse tissues as well as primary T cells from human blood, portraying the native-state mitochondrial proteomic characteristics, and unveiled a set of hidden mitochondrial proteins in human proteome. Furthermore, CAT-S allows quantitative analysis of the in situ proteomic perturbations on dysfunctional tissue samples, exampled by diabetic mouse kidneys, and revealed the alterations of lipid metabolism machinery that drive the disease progression. Given the advantages of non-genetic operation, generality, efficiency as well as spatiotemporal resolution, CAT-S may open new avenues as a proximity labeling strategy for in situ investigation of subcellular proteomic landscape of primary living samples that are otherwise inaccessible.
HostingRepositoryPRIDE
AnnounceDate2024-05-24
AnnouncementXMLSubmission_2024-05-24_01:23:51.478.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZiqi Liu
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-28 13:50:44ID requested
12024-05-24 01:23:52announced
22024-10-22 06:42:32announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41467-024-46985-3;
Liu Z, Guo F, Zhu Y, Qin S, Hou Y, Guo H, Lin F, Chen PR, Fan X, Bioorthogonal photocatalytic proximity labeling in primary living samples. Nat Commun, 15(1):2712(2024) [pubmed]
Keyword List
submitter keyword: proximity labeling, mitochondria, bioorthogonal chemistry,photocatalysis
Contact List
Xinyuan Fan
contact affiliationPeking University
contact email1906885003@pku.edu.cn
lab head
Ziqi Liu
contact affiliationCollege of Chemistry and Chemical Engineering, Peking University
contact emailzqliu7@pku.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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