PXD045787 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli |
Description | The biological significance of circular RNAs remains largely unexplored due to the lack of loss-of-function animal models. In this study, we focused on circTulp4, a highly abundant circRNA that is enriched in the brain and synaptic compartments. We created a circTulp4-deficient (CD) mouse model and conducted a comprehensive phenotypic analysis. To test whether the electrophysiological phenotype observed in CD mice was explained by an alteration in the levels of synaptic proteins, we prepared pure synaptosomal fractions and conducted a quantitative proteomic analysis. Label-free quantification of CD and wildtype synaptic proteomes was performed by using an ion mobility-enhanced data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMSE). We quantified >1800 proteins and concluded that the protein composition of the synaptosomal compartment is preserved in CD mice. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:36:04.172.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Olaf Jahn |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-28 11:25:58 | ID requested | |
1 | 2024-04-15 05:14:33 | announced | |
⏵ 2 | 2024-10-22 06:36:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: synaptosomes, label-free proteomics, mass spectrometry, CircTulp4, phenotyping, neurophysiology,Circular RNA |
Contact List
Olaf Jahn |
contact affiliation | Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany |
contact email | jahn@mpinat.mpg.de |
lab head | |
Olaf Jahn |
contact affiliation | Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany |
contact email | jahn@mpinat.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD045787 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045787
- Label: PRIDE project
- Name: A brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli