PXD045761-1

PXD045761 is an original dataset announced via ProteomeXchange.

Title	Identification of phosphorylation sites in human IGF2BP1 by immunoprecipitation coupled to mass spectrometry
Description	Many RNA-binding proteins are regulated by posttranslational modifications, most commonly phosphorylation. IGF2BP1 is an RNA-binding protein, which regulates protein stability, translation and localization. Moreover, it was proposed to regulate mRNA stability and translation during proteotoxic stress. Here, we used immunoprecipitation coupled to mass spectrometry to identify phosphorylation sites in IGF2BP1 under control conditions and under conditions where mammalian cells were subjected to proteotoxic stress.
HostingRepository	PRIDE
AnnounceDate	2024-11-13
AnnouncementXML	Submission_2024-11-13_03:18:47.367.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Hollenstein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 02:19:03	ID requested
⏵ 1	2024-11-13 03:18:48	announced

10.1038/s41467-024-53400-4;

Hornegger H, Anisimova AS, Muratovic A, Bourgeois B, Spinetti E, Niedermoser I, Covino R, Madl T, Karag, ö, z GE, IGF2BP1 phosphorylation in the disordered linkers regulates ribonucleoprotein condensate formation and RNA metabolism. Nat Commun, 15(1):9054(2024) [pubmed]

submitter keyword: Q Exactive HF-X, human,IGF2BP1, proteotoxic stress, HEK293T, IGF2BP3

Gülsün Elif Karagöz
contact affiliation	Max Perutz Labs, Vienna BioCenter Campus (VBC), 1030 Vienna, Austria Medical University of Vienna, Center for Medical Biochemistry, 1030 Vienna, Austria
contact email	elif.karagoez@univie.ac.at
lab head
David Hollenstein
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 7, Vienna 1030, Austria
contact email	hollenstein.david@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD045761

PRIDE project URI

• PRIDE
  1. PXD045761
     1. Label: PRIDE project
     2. Name: Identification of phosphorylation sites in human IGF2BP1 by immunoprecipitation coupled to mass spectrometry