PXD045756-2

PXD045756 is an original dataset announced via ProteomeXchange.

Title	Neutrophil depletion experiment in ischemia reperfusion mouse model
Description	To elucidate the role of neutrophils in the molecular alterations observed after I/R, we performed an experiment of I/R in neutrophil-depleted mice. Neutrophil depletion was performed by anti-Ly6G injection and IgG was used as vehicle. We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the mouse heart tissue at 24h after I/R. Neutrophil depletion was performed in 3 mice by Ly6G injection 24h and 48 h prior to I/R and sacrificed 24 hours after reperfusion. A second group consisting on 3 mice that were injected with IgG antibody as vehicle, and subjected to I/R and sacrificed 24h after reperfusion. A third group of 3 mice with no injection were also sacrificed 24h after reperfusion. 12 additional mice from the three previous groups were sacrificed without I/R intervention: control without injection (n=6), IgG injection (n=3) and Ly6G injection (n=3). Whole hearts tissue was collected for proteomics evaluation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:23.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inmaculada Jorge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 00:01:49	ID requested
1	2024-05-24 01:57:09	announced
⏵ 2	2024-10-22 06:42:31	announced	2024-10-22: Updated project metadata.

10.3390/antiox13010106;

Binek A, Castans C, Jorge I, Bagwan N, Rodr, í, guez JM, Fern, á, ndez-Jim, é, nez R, Gal, á, n-Arriola C, Oliver E, G, ó, mez M, Clemente-Morag, ó, n A, Ibanez B, Camafeita E, V, á, zquez J, Oxidative Post-translational Protein Modifications upon Ischemia/Reperfusion Injury. Antioxidants (Basel), 13(1):(2024) [pubmed]

submitter keyword: ischemia reperfusion, mouse, heart tissue,quantitative proteomics

Jesús Vázquez Cobos
contact affiliation	Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain
contact email	jesus.vazquez@cnic.es
lab head
Inmaculada Jorge
contact affiliation	Vascular Biology and Inflammation
contact email	ijorge@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045756

PRIDE project URI

• PRIDE
  1. PXD045756
     1. Label: PRIDE project
     2. Name: Neutrophil depletion experiment in ischemia reperfusion mouse model