PXD045743-2

PXD045743 is an original dataset announced via ProteomeXchange.

Title	Nitrogen-fixation in the widely distributed model marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni
Description	The global significance of marine non-cyanobacterial diazotrophs, notably heterotrophic bacterial diazotrophs (HBDs), has become increasingly clear. Understanding N2 fixation rates for these largely uncultured organisms poses a challenge due to uncertain growth requirements and complex nitrogenase regulation. We identified Candidatus Thalassolituus haligoni as an Oceanospirillales member, closely related to other significant γ-proteobacterial HBDs. Pangenome analysis reinforces this classification, indicating the isolate belongs to the same species as the uncultured metagenome-assembled genome Arc-Gamma-03. Analysis of the nifH gene in amplicon sequencing libraries reveals the extensive distribution of Cand. T. haligoni across the Pacific, Atlantic and Arctic Oceans. Through combined proteomic analysis and N2 fixation rate measurements, we confirmed the isolate’s capacity for nitrate independent N2 fixation, although a clear understanding of nitrogenase regulation remains unclear. Overall, our study highlights the significance of Cand. T. haligoni as the first globally distributed, cultured model species within the understudied group of Oceanospirillales, and γ-HBDs in general.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:14.950.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045743
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elden Rowland
SpeciesList	scientific name: environmental samples
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 11:58:21	ID requested
1	2024-08-10 12:22:34	announced
⏵ 2	2024-10-22 06:54:18	announced	2024-10-22: Updated project metadata.

10.1126/sciadv.adn1476;

10.6019/PXD045743;

submitter keyword: Nitrogen-fixation, diazotroph, Candidatus Thalassolituus haligoni, proteome

Erin Bertrand
contact affiliation	Professor Biology, Dalhousie University
contact email	erin.bertrand@dal.ca
lab head
Elden Rowland
contact affiliation	Dalhousie University
contact email	erowland@dal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045743
     1. Label: PRIDE project
     2. Name: Nitrogen-fixation in the widely distributed model marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni