PXD045738-1

PXD045738 is an original dataset announced via ProteomeXchange.

Title	Dysferlin and its role in cardiac disease
Description	To explore the proteomic interactome of Dysferlin in the heart, we used recombinant expression of N-terminally Twin-Strep II-tagged full-length human Dysferlin to establish an in vitro binding assay. Isolated mouse heart membrane fractions were incubated with Dysferlin-coupled beads under four different detergent conditions. Dysferlin pull-down eluates were analyzed by label-free quantification using an ion mobility-enhanced data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMSE).
HostingRepository	PRIDE
AnnounceDate	2024-07-14
AnnouncementXML	Submission_2024-07-14_03:09:31.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 09:33:28	ID requested
⏵ 1	2024-07-14 03:09:31	announced
2	2024-10-22 06:49:46	announced	2024-10-22: Updated project metadata.

10.1161/CIRCRESAHA.124.324588;

submitter keyword: mouse heart tissue,Dysferlin, label-free quantification, data-independent acquisition, interactome

Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD045738

PRIDE project URI

• PRIDE
  1. PXD045738
     1. Label: PRIDE project
     2. Name: Dysferlin and its role in cardiac disease