PXD045732-2

PXD045732 is an original dataset announced via ProteomeXchange.

Title	Lachesis muta snake venom peptidome
Description	Snake venoms are known to be major sources of peptides with different pharmacological properties. In this study, we comprehensively explored the venom peptidomes of three specimens of Lachesis muta, the largest venomous snake in South America, using mass spectrometry techniques. The analysis revealed 19 main chromatographic peaks common to all specimens. A total of 151 peptides were identified, including 69 from a metalloproteinase, 58 from the BPP-CNP precursor, and 24 from a L-amino acid oxidase. To our knowledge, 126 of these peptides were reported for the first time in this work, including a new SVMP-derived peptide fragment, Lm-10a. Our findings highlight the dynamic nature of toxin maturation in snake venoms, driven by proteolytic processing, post-translational modifications, and cryptide formation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:11.471.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Lachesis muta; NCBI TaxID: 8752;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 07:05:29	ID requested
1	2023-10-23 14:01:23	announced
⏵ 2	2023-11-14 09:06:12	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:07:13	announced	2024-10-22: Updated project metadata.

Ito LT, Miyamoto JG, Sant'Anna SS, Grego KF, Tanaka-Azevedo AM, Tashima AK, Unveiling the peptidome diversity of Lachesismuta snake venom: Discovery of novel fragments of metalloproteinase, l-amino acid oxidase, and bradykinin potentiating peptides. Biochem Biophys Res Commun, 683():149090(2023) [pubmed]

submitter keyword: peptidomics, bradykinin potentiating peptides, mass spectrometry,Lachesis muta, snake venom, snake venom metalloproteinases

Alexandre Keiji Tashima
contact affiliation	Department of biochemistry, UNIFESP, Brazil.
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045732

PRIDE project URI

• PRIDE
  1. PXD045732
     1. Label: PRIDE project
     2. Name: Lachesis muta snake venom peptidome