PXD045729 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human serum proteomics reveal molecular signature after one night of sleep deprivation |
Description | Study Objectives: Sleep deprivation is highly prevalent and caused by conditions such as night shift work or illnesses like obstructive sleep apnea. Compromised sleep is proposed to play a role in several cardiovascular, immune related and neurodegenerative disorders. We recently published human serum proteome changes after a simulated night shift. This study aimed to further explore changes in the human blood serum after 6h of sleep deprivation at night by proteomics and systems biological databases. Methods: Human blood serum samples from 8 self-declared healthy females were analyzed using mass spectrometry and high-pressure liquid chromatography. Each subject was their own control, and two samples were taken from each subject, the first one after 6h of sleep at night and the second one after 6h of sleep deprivation the following night. Biological databases and bioinformatic software were used for systems biological analyzes and comparative analysis with other published sleep-related datasets. Results: Of 494 proteins, 66 were found to be differentially expressed after 6h of sleep deprivation at night. Functional enrichment analysis revealed associations of these proteins with several biological functions related to the regulation of cellular processes like protein- and ion-binding connected to platelet degranulation and blood coagulation, as well as associations with different curated gene sets. Conclusions: This study presents serum proteomic changes after 6h of sleep deprivation, supports previous findings that only 6h of sleep deprivation affects several biological processes and revealed a molecular signature of protein changes related to pathological conditions like altered coagulation and platelet function, impaired lipid and immune function and cancer. Keywords: Human blood serum, proteomics, sleep deprivation, cellular stress, functional enrichment analysis |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-27 |
AnnouncementXML | Submission_2024-06-27_06:38:33.386.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Even Birkeland |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-27 06:10:26 | ID requested | |
⏵ 1 | 2024-06-27 06:38:33 | announced | |
Publication List
Keyword List
submitter keyword: Human blood serum, proteomics, sleep deprivation, functional enrichment analysis, cellular stress |
Contact List
Even Birkeland |
contact affiliation | Department of genetics and bioinformatics, Norwegian Institute of Health |
contact email | even.birkeland@fhi.no |
lab head | |
Even Birkeland |
contact affiliation | Institute of biomedicine, University of Bergen and Norwegian Institute of Public Health |
contact email | even.birkeland@uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045729
- Label: PRIDE project
- Name: Human serum proteomics reveal molecular signature after one night of sleep deprivation