PXD045728-1

PXD045728 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparison of SUMOylated proteins between wild-type and EMA2 KO Tetrahymena cells
Description	Small RNAs target their complementary chromatin regions for gene silencing through nascent long non-coding RNAs (lncRNAs). In programmed DNA elimination of the ciliated protozoan Tetrahymena, the interaction between Piwi-associated small RNA (scnRNAs) and the lncRNA transcripts from the somatic genome has been proposed to induce target-directed scnRNA degradation (TDSD) and scnRNAs not targeted for TDSD later target the germline-limited sequences for DNA elimination. In this study, we show that the SUMO E3 ligase Ema2 is required for the accumulation of lncRNAs from somatic genome, and thus for TDSD and completing DNA elimination to make viable sexual progeny. Ema2 interacts with the SUMO E2 conjugating enzyme Ubc9 and enhances SUMOylation of the transcription regulator Spt6. We further show that Ema2 promotes the association of Spt6 and RNA polymerase II to chromatin. These results suggest that Ema2-directed SUMOylation actively promotes the lncRNA transcription that is a prerequisite for communication between the genome and small RNAs.
HostingRepository	MassIVE
AnnounceDate	2023-09-27
AnnouncementXML	Submission_2023-09-27_07:52:31.609.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform
SpeciesList	scientific name: Tetrahymena thermophila; NCBI TaxID: 5911;
ModificationList	Acetyl; Carbamidomethyl; Oxidation
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 05:17:26	ID requested
⏵ 1	2023-09-27 07:52:32	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: sumo, Tetrahymena

submitter keyword: sumo, Tetrahymena

Contact List

Kazufumi MOCHIZUKI
contact affiliation	Institute of Human Genetics, CNRS-Univ. Montpellier, Montpellier
contact email	Kazufumi.mochizuki@cnrs.fr
lab head
Functional Proteomics Platform
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092977/