PXD045725-1

PXD045725 is an original dataset announced via ProteomeXchange.

Title	Structural basis of RNA-induced autoregulation of the DExH-type RNA helicase maleless
Description	RNA unwinding by DExH-type helicases underlies most RNA metabolism and function. It remains unresolved if and how the basic unwinding reaction of helicases is regulated by auxiliary domains. We explored the interplay between the RecA and auxiliary domains of the RNA helicase maleless (MLE) from Drosophila, using a suite of structural and functional studies. We discovered that MLE exists in a dsRNA bound open conformation and the auxiliary dsRBD2 domain aligns the substrate RNA with the accessible helicase tunnel. In an ATP-dependent manner, dsRBD2 associates with the helicase module, leading to tunnel closure around ssRNA. Furthermore, our structures provide a rationale for blunt ended dsRNA unwinding and 3’-5’ translocation by MLE. Structure-based MLE mutations confirm the functional relevance of our model for RNA unwinding. Our findings contribute to our understanding of fundamental mechanics of auxiliary domains in DExH helicase MLE which serves as model for its human ortholog and potentially therapeutic target, DHX9/RHA.
HostingRepository	PRIDE
AnnounceDate	2023-11-27
AnnouncementXML	Submission_2023-11-27_01:26:26.190.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Kiss
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 01:41:55	ID requested
⏵ 1	2023-11-27 01:26:26	announced
2	2024-10-22 06:15:53	announced	2024-10-22: Updated project metadata.

10.1016/J.MOLCEL.2023.10.026;

submitter keyword: Helicase, QexactiveHF, CL-MS, MLE, RNA, CX-MS, crosslinking, Drosophila melanogaster

Janosch Hennig
contact affiliation	1) Structural and Computational Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117, Heidelberg, Germany 2) Chair of Biochemistry IV, Biophysical Chemistry, University of Bayreuth, Bayreuth, Germany
contact email	janosch.hennig@embl.de
lab head
Anna Kiss
contact affiliation	Ludwig-Maximilians-Universität, München
contact email	anna.kiss@bmc.med.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD045725

PRIDE project URI

• PRIDE
  1. PXD045725
     1. Label: PRIDE project
     2. Name: Structural basis of RNA-induced autoregulation of the DExH-type RNA helicase maleless