PXD045719-2

PXD045719 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Microglial Rac1 is essential for experience-dependent brain plasticity and cognitive performance
Description	Here, we used conditional cell-specific gene targeting in mice with multi-omics approaches, and demonstrated that the RhoGTPase Rac1 was an essential requirement for the microglia to sense and interpret the brain microenvironment, and for crucial microglia-synapse crosstalk driving experience-dependent plasticity. Phosphoproteomics profiling detected a large modulation of RhoGTPase signaling, predominantly of Rac1, in microglia of mice exposed to an environmental enrichment protocol. Ablation of microglial Rac1 affected pathways involved in microglia-synapse communication, disrupted experience-dependent synaptic remodeling, and blocked the gains in learning, memory and sociability induced by environmental enrichment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:07:01.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tiago Almeida
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-26 20:11:30	ID requested
1	2023-10-23 06:12:27	announced
⏵ 2	2024-10-22 06:07:02	announced	2024-10-22: Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: microglia
synaptosomes
glia-neuron interaction
Highthroughput phosphoproteomics
synaptic plasticity

submitter keyword: microglia

synaptosomes

glia-neuron interaction

Highthroughput phosphoproteomics

synaptic plasticity

Contact List

João Bettencourt Relvas
contact affiliation	Glial Cell Biology Lab, i3s - Instituto de Investigação e Inovação em Saúde, Porto, Portugal
contact email	jrelvas@i3s.up.pt
lab head
Tiago Almeida
contact affiliation	i3s - Instituto de Investigação e Inovação em Saúde da Universidade do Porto/Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto
contact email	tiago.almeida@i3s.up.pt
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045719
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045719

PRIDE project URI

Repository Record List

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