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PXD045716-1

PXD045716 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative nuclear proteomic analysis between undifferentiated and differentiation-initiated mouse spermatogonial stem cell cultures
DescriptionMouse spermatogonial stem cells (SSCs) continuously self-renew on the feeder layers in serum-free culture medium supplemented with glial cell line-derived neurotrophic factor and fibroblast growth factor 2. To identify novel nuclear proteins involved in SSC maintenance, comparative proteomic profiling of nuclear proteins was performed between self-renewing and differentiation-initiated SSCs in culture. The self-renewing SSC cultures were established from C57BL/6 mouse testes. Nuclear fractions from self-renewing SSC cultures treated with ethanol as a vehicle control (spermatogonial stem cells) and differentiation-initiated SSC cultures treated with 0.3 μM retinoic acid for 24 h (spermatogonial progenitor cells) were isolated for proteomic analysis.
HostingRepositoryjPOST
AnnounceDate2024-09-27
AnnouncementXMLSubmission_2024-09-26_08:00:07.329.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD045716
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKazue Kakiuchi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-26 18:31:27ID requested
12024-09-26 08:00:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: spermatogonial stem cells, spermatogonia, spermatogenesis, testis
Contact List
Hiroshi Kubota
lab head
Kazue Kakiuchi
contact affiliationKITASATO UNIVERSITY
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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