PXD045716-1

PXD045716 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative nuclear proteomic analysis between undifferentiated and differentiation-initiated mouse spermatogonial stem cell cultures
Description	Mouse spermatogonial stem cells (SSCs) continuously self-renew on the feeder layers in serum-free culture medium supplemented with glial cell line-derived neurotrophic factor and fibroblast growth factor 2. To identify novel nuclear proteins involved in SSC maintenance, comparative proteomic profiling of nuclear proteins was performed between self-renewing and differentiation-initiated SSCs in culture. The self-renewing SSC cultures were established from C57BL/6 mouse testes. Nuclear fractions from self-renewing SSC cultures treated with ethanol as a vehicle control (spermatogonial stem cells) and differentiation-initiated SSC cultures treated with 0.3 μM retinoic acid for 24 h (spermatogonial progenitor cells) were isolated for proteomic analysis.
HostingRepository	jPOST
AnnounceDate	2024-09-27
AnnouncementXML	Submission_2024-09-26_08:00:07.329.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045716
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kazue Kakiuchi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-26 18:31:27	ID requested
⏵ 1	2024-09-26 08:00:07	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: spermatogonial stem cells, spermatogonia, spermatogenesis, testis

submitter keyword: spermatogonial stem cells, spermatogonia, spermatogenesis, testis

Contact List

Hiroshi Kubota
lab head
Kazue Kakiuchi
contact affiliation	KITASATO UNIVERSITY
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002202/