PXD045705 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Insights into enhancing Centella asiatica organ cell biofactories via hairy root protein profiling |
Description | The objectives of this study encompassed a thorough exploration of the potential implications of protein profiling in hairy roots, specifically focusing on optimizing and enhancing C. asiatica organ cell biofactories. In this pursuit, we categorized established C. asiatica hairy root lines according to their capacity for centelloside production, classifying them into HIGH, MID, or LOW categories. For comparative analysis, wild adventitious (Adv) roots were extracted from in vitro C. asiatica seedlings and cultivated in solid MS medium at 25°C in complete darkness, serving as control specimens. This meticulous, label-free proteomic analysis enabled the successful identification of several proteins. Our research substantially builds upon and extends the findings presented by Alcalde et al. (2022) (DOI: 10.3389/fpls.2022.1001023). In their study, distinctive morphological and metabolic variations were noted among different C. asiatica hairy root lines. Such differences are presumably attributable to the random incorporation of a selective set of genes from the T-DNA, with particular emphasis on the rol and aux genes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_08:15:05.230.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Diego Alberto Hidalgo Martinez |
SpeciesList | scientific name: Centella asiatica; NCBI TaxID: 48106; |
ModificationList | iodoacetamide derivatized residue |
Instrument | 6550 iFunnel Q-TOF LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-26 10:50:53 | ID requested | |
⏵ 1 | 2024-01-26 08:15:06 | announced | |
Publication List
Alcalde MA, Hidalgo-Martinez D, Bru Mart, í, nez R, Sell, é, s-Marchart S, Bonfill M, Palazon J, organ cell biofactories via hairy root protein profiling. Front Plant Sci, 14():1274767(2023) [pubmed] |
10.3389/fpls.2023.1274767; |
Keyword List
submitter keyword: Centella asiatica, plant biotechnology, molecular farming, Centelloside production,Rhizobium rhizogenes, biomarkers |
Contact List
Diego A. Hidalgo |
contact affiliation | Dpt. Biologia, Sanidad y Medio ambiente Fac. Farmacia y Ciencias de la Alimentacion Univ. Barcelona Av.Joan XIII, 27-31 08028 Barcelona SPAIN |
contact email | dhidalgo@ub.edu |
lab head | |
Diego Alberto Hidalgo Martinez |
contact affiliation | University of Barcelona |
contact email | dhidalgo@ub.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045705
- Label: PRIDE project
- Name: Insights into enhancing Centella asiatica organ cell biofactories via hairy root protein profiling