PXD045694 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enhancer bound proteome of intronic Ig Emu enhancer with MAR element deletion control bait |
Description | The specificity of humoral immune responses depends on the functional rearrangement and expression of only one allele of immunoglobulin (Ig) genes. Here, we analyzed the comprehensive proteome of the murine Ig Emu enhancer, which governs the rearrangement and expression of the Ig mu heavy chain allele. By mass spectrometry of proteins bound at wild type versus mutant Emu enhancers, we identified Emu-binding proteins and associated multi-protein complexes. We found that the MSL/MOF complex, a regulator of gene dosage compensation in flies, binds Emu via transcription factor YY1 and facilitates Emu-driven chromatin looping and promoter interaction. Msl2 gene knockout in primary pre-B cells or Mof heterozygosity in mice reduced mu gene expression. In this data set we compare proteins binding to the wild-type Emu versus a DNA bait control for which the 5’ and 3’ MAR (matrix attachment region) sequences were deleted (and substituted with the murine Ig mu locus DNA flanking the MARs).The Delta MAR mutant abrogates binding of proteins recognizing MAR elements. SILAC quantitative proteomics was employed in a label swap approach incubating wild-type and control DNA with labeled and non-labled protein extracts, respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-25 |
AnnouncementXML | Submission_2024-10-25_03:38:43.172.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gerhard Mittler |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-26 05:49:51 | ID requested | |
⏵ 1 | 2024-10-25 03:38:43 | announced | |
Publication List
Keyword List
submitter keyword: AP-MS, reverse ChIP, LC-MS, SILAC, DNA protein interaction, matrix attachment region,Immunoglobulin Ig Emu enhancer |
Contact List
Gerhard Mittler |
contact affiliation | Max Planck Institute of Immunobiology and Epigenetics Proteomics Unit Stuebeweg 51 79108 Freiburg Germany |
contact email | mittler@ie-freiburg.mpg.de |
lab head | |
Gerhard Mittler |
contact affiliation | Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045694
- Label: PRIDE project
- Name: Enhancer bound proteome of intronic Ig Emu enhancer with MAR element deletion control bait