PXD045676 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Intracellular targets of the opioids |
Description | Opioids are powerful analgesics but carry many contingencies that can lead to opioid-use disorder. Significant energy has been invested in understanding the molecular actions of the opioid alkaloids, particularly their relationship with the opioid receptors at the cell surface. However, there is growing awareness of the opioid-receptor-independent and intracellular actions of opioids that may contribute to their overall pharmacology. The investigation of these interactions is stymied by a lack of relevant opioid chemical tools to probe intracellular actions of the opioids. To investigate the intracellular interactions of the opioids, we designed and developed two opioid probes: photo-click morphine (PCM-2) as a photo-affinity probe for morphine and dialkynyl-acetyl morphine (DAAM) as a metabolic acetate reporter for heroin. Application of these probes to SH-SY5Y, HEK293T, and U2OS cells revealed that PCM-2 and DAAM primarily localize to the lysosome by confocal microscopy and chemical proteomics studies. Interaction site identification by mass spectrometry revealed the mitochondria phosphate carrier protein, solute carrier family 25 member 3, SLC25A3, and histone H2B as acetylation targets of DAAM. This study provides the first chemical probes for the study of the intracellular targets of opioid alkaloids. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-07 |
AnnouncementXML | Submission_2024-05-07_13:34:33.920.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christina Woo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-25 17:16:53 | ID requested | |
⏵ 1 | 2024-05-07 13:34:34 | announced | |
Publication List
10.1002/cbic.202300841; |
Duque MAL, Vallavoju N, Zhang T, Yvon R, Pan YX, Woo CM, Photo-affinity and Metabolic Labeling Probes Based on the Opioid Alkaloids. Chembiochem, 25(6):e202300841(2024) [pubmed] |
Keyword List
submitter keyword: heroin, morphine,opioids, LC-MS/MS |
Contact List
Christina M. Woo |
contact affiliation | Harvard University Chemistry and Chemical Biology Department |
contact email | cwoo@chemistry.harvard.edu |
lab head | |
Christina Woo |
contact affiliation | Harvard University |
contact email | cwoo@chemistry.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD045676 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045676
- Label: PRIDE project
- Name: Intracellular targets of the opioids