PXD045672-1

PXD045672 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Physiological and Cognitive Changes after Treatments of Cyclophosphamide, Methotrexate, and Fluorouracil: Implications of the Gut Microbiome and Depressive-Like Behavior
Description	Chemotherapy induced cognitive impairment, colloquially referred to as chemobrain, is a poorly understood phenomenon affecting a highly variable proportion of patients with breast cancer. Inflammation, a condition associated with many pathologies, is considered to be a potential mechanism behind chemobrain. Here we investigate the association between anxiety and despair-like behaviors in mice treated with cyclophosphamide, methotrexate, and fluorouracil along with host histological, proteomic, gene expression, and gut microbial responses. Forced swim and sociability tests were used to evaluate depression and despair like behaviors. Tandem mass tag proteomics approach was used to assess changes in neural protein network of the amygdala and hippocampus. While quantitative reverse transcription polymerase chain reaction, we evaluated changes in intestinal gap junction markers. Finally, the composition of gut microbiota was assessed through 16S rRNA gene sequencing. We have observed that CMF induced social and despair like behavior in mice 96 hours following treatment. Proteomic analysis identified changes various proteins related to progressive neurological disease, working memory deficit, primary anxiety disorder and gene expression revealed increases in NDMA and AMPA receptors in both the hippocampus and the amygdala as a result of CMF treatment. Finally, we observed immediate changes in the microbial population after chemotherapy treatment, with a notable increase in the mucinophilic bacteria Akkermansia mucinophila.
HostingRepository	MassIVE
AnnounceDate	2023-09-26
AnnouncementXML	Submission_2023-09-26_05:53:22.257.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephanie Byrum
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-25 13:59:38	ID requested
⏵ 1	2023-09-26 05:53:22	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: hippocampus, chemotherapy, microbiome

submitter keyword: hippocampus, chemotherapy, microbiome

Contact List

Antino Allen
contact affiliation	University of Arkansas for Medical Sciences
contact email	arallen@uams.edu
lab head
Stephanie Byrum
contact affiliation	University of Arkansas for Medical Sciences
contact email	sbyrum@uams.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092957/