PXD045646-1
PXD045646 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of PROSER2 overexpressing cells |
| Description | Pancreatic Ductal Adenocarcinoma (PDAC) is diagnosed at an advanced stage in around 70% of patients and has a dismal 5-year survival rate of less than 11%. This dismal prognosis arises not only due to the absence of diagnostic markers and molecular targets but also due to its aggressive metastatic characteristics. In this research, we took a departure from conventional proteomic studies to identify new molecular targets for pancreatic cancer. We chose uPE1 from an extensive pancreatic cancer proteomic research database and focused our investigation on Proline and Serine Rich 2 (PROSER2), a protein that ranked high in both the cell membrane and cytoplasm. While PROSER2 has been reported to be associated with tumor progression, its exact function remained unclear. In our study using cell lines and patient-derived cells, PROSER2 exhibited a higher expression pattern primarily in cells derived from primary tumors compared to those from metastatic tissues. Overexpression of PROSER2 significantly reduced the metastatic ability of cancer cells, whereas its suppression had the opposite effect. Through proteomic analysis, we discovered that PROSER2 interacts with STK25 and PDCD10, proteins associated with cancer metastatic signaling, and this interaction was confirmed in PROSER2-overexpressing MIAPACA-2 cells. Immunocytochemistry in patient-derived xenograft (PDOX) cells further verified the co-localization of endogenous PROSER2 with STK25 and PDCD10 in the cell membrane and cytoplasm. Taken together, this study is the first to describe the novel role of PROSER2 in antagonizing invasion and proliferation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-11-16 |
| AnnouncementXML | Submission_2023-11-16_07:39:15.224.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Yeji Yang |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-25 04:37:07 | ID requested | |
| ⏵ 1 | 2023-11-16 07:39:15 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Cholangiocarcinoma, Human Proteome Project, LC-MS/MS |
Contact List
| Heeyoun Hwang | |
|---|---|
| contact affiliation | Korea Basic Science Institute |
| contact email | heeyounh@kbsi.re.kr |
| lab head | |
| Yeji Yang | |
| contact affiliation | Korea Basic Science Institute |
| contact email | yangyj@kbsi.re.kr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092953/ |
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