PXD045608-1

PXD045608 is an original dataset announced via ProteomeXchange.

Title	Ftdc1/2, maternal-effect genes required for methylation maintenance and embryonic development
Description	The oocyte serves the different purpose of transmitting the maternal genome and factors that are critical for post-fertilization events. Through the screening of oocyte-specific factors, here we identify two hitherto uncharacterized murine genes, ferritin domain containing 1 and 2 (Ftdc1 and Ftdc2), as novel maternal-effect genes. Genetic ablation of Ftdc1 or Ftdc2 causes postimplantation defects and female infertility, despite having little impact on oogenesis and preimplantation development. Nevertheless, post-transcriptional events in these mutant oocytes are disrupted. Importantly, FTDC1 or FTDC2 depletion induces the progressive loss of genomic methylation in early embryos. This loss correlates with a marked reduction in oocyte-specific DNA methyltransferase 1 (DNMT1o) protein, due to its increased degradation via the ubiquitin-proteasome pathway. Mechanistically, we reveal that FTDC1, FTDC2 and DNMT1o form a complex by direct interactions, thereby stabilizing each other. Moreover, comparison with DNA and histone methylation profiles in Dnmt1-null embryos indicates the functional uniqueness of FTDC1/2. These findings demonstrate that maternal FTDC1 and FTDC2 are crucial players in maintaining DNA methylation during embryogenesis and determining female fertility, offering new insights into the epigenetic control of mammalian development.
HostingRepository	PRIDE
AnnounceDate	2025-10-29
AnnouncementXML	Submission_2025-10-28_20:30:08.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yueshuai Guo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 19:25:01	ID requested
⏵ 1	2025-10-28 20:30:09	announced

10.1038/s41418-025-01518-3;

Li C, Li J, Du S, Ma Y, Guo Y, Zhang X, Wang B, Zhu S, An H, Chen M, Guo J, Han L, Ge J, Qian X, Schedl T, Guo X, Wang Q, FTDC1/2, oocyte-specific cofactors of DNMT1 required for epigenetic regulation and embryonic development. Cell Death Differ, 32(11):2093-2110(2025) [pubmed]

submitter keyword: embryo, DNA methyltransferase, proteomics, epigenetics, maternal factor, mouse,Oocyte

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine and Offspring Health
contact email	guo_xuejiang@njmu.edu.cn
lab head
Yueshuai Guo
contact affiliation	Nanjing Medical University
contact email	gys_706@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045608
     1. Label: PRIDE project
     2. Name: Ftdc1/2, maternal-effect genes required for methylation maintenance and embryonic development