PXD045581 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of RNAseT2 interactome in C2C12 cells |
| Description | Muscle stem cells (MuSCs) fuse to form multinucleated cells to repair skeletal muscle after injury. While adult MuSCs express a cell autonomous myogenesis program, in vivo investigations demonstrated that the MuSC environment is required for efficient adult myogenesis and muscle regeneration. Particularly, anti-inflammatory/restorative macrophages stimulate MuSC differentiation and fusion, although the molecular mechanisms involved are still largely unknown. Here, we show that restorative macrophages secrete ribonuclease T2 (RNAseT2) which specifically stimulates MuSC fusion. Co-immuprecipitation coupled to mass spectrometry-based proteomics identified Ste20-like kinase (SLK) as binding partner of RNAseT2. SLK triggered the phosphorylation-mediated activation of N-WASP, allowing actin remodeling at the time of MuSC fusion. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-15 |
| AnnouncementXML | Submission_2026-06-14_16:14:23.156.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD045581 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Yohann Couté |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-09-22 02:12:42 | ID requested | |
| ⏵ 1 | 2026-06-14 16:14:23 | announced | |
Publication List
| 10.6019/PXD045581; |
| Weiss-Gayet M, Juban G, Le Moal E, Moretta A, Farnetani C, Gobet C, Guillemaud J, Le Bihan MC, Shoseyov O, Adrait A, Ternka K, Boespflug-Tanguy O, Kettwig M, Cout, é Y, Mounier R, Acquati F, Knight RD, Chazaud B, Restorative macrophage-derived RNAseT2 stimulates muscle stem cell fusion via an SLK/N-WASP/actin bundling dependent axis. Nat Commun, 17(1):(2026) [pubmed] |
| 10.1038/s41467-026-71990-z; |
Keyword List
| submitter keyword: co-immunoprecipitation,Skeletal mucle injury, Muscle stem cells, ribonuclease T2, nanoLC-MS/MS |
Contact List
| Yohann Couté |
|---|
| contact affiliation | EDyP |
| contact email | yohann.coute@cea.fr |
| lab head | |
| Yohann Couté |
|---|
| contact affiliation | EDyP |
| contact email | yohann.coute@cea.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD045581 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045581
- Label: PRIDE project
- Name: Characterization of RNAseT2 interactome in C2C12 cells
