PXD045574-1

PXD045574 is an original dataset announced via ProteomeXchange.

Title	Unnatural lactam production by reprogrammed modular polyketide synthases in Pseudomonas putida
Description	In recent years, type I polyketide synthases (PKSs) has emerged as an enticing platform for the production of novel molecules by reconstituting their modules, expanding the chemical landscape beyond the limitations of conventional metabolic engineering. This study reports a retrobiosynthesis approach to produce five-carbon lactam and its derivatives through the heterologous expression of reprogrammed PKS within Pseudomonas putida KT2440, a widely employed organism for the sustainable commodity chemical production. Combining with host optimization to prevent the catabolism of target product and engineering to facilitate the production of unique acyl-CoA extender units for PKS, this strategy enables mg · l−1 scale microbial production of unnatural lactams, even utilizing plant biomass hydrolysate as a starting material. The resulting novel nylons, derived from the polymerization of these unexplored monomers, hold promise for advancing textile materials, providing sustainable alternatives with potential across various industrial applications.
HostingRepository	PRIDE
AnnounceDate	2025-01-22
AnnouncementXML	Submission_2025-01-22_13:25:48.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-21 16:36:58	ID requested
⏵ 1	2025-01-22 13:25:49	announced

Dataset with its publication pending

submitter keyword: Pseudomonas putida,Unatural lactam,Polyketide synthases

Christopher J. Petzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045574
     1. Label: PRIDE project
     2. Name: Unnatural lactam production by reprogrammed modular polyketide synthases in Pseudomonas putida