PXD045568-2

PXD045568 is an original dataset announced via ProteomeXchange.

Title	In-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.
Description	Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensional gel electrophoresis (2-DE) pattern between asthmatic and helthy horses´sera. Differentially regulated spots were cut from a 2-D gel and used for in-gel digestion followed by LC-MS to identify possible antigens.
HostingRepository	PanoramaPublic
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_17:05:19.642.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniela Volke
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; scientific name: Dermatophagoides pteronyssinus; NCBI TaxID: 6956;
ModificationList	Carbamidomethyl; Oxidation
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-21 11:18:18	ID requested
1	2023-11-30 14:09:01	announced
⏵ 2	2024-01-26 17:05:20	announced	: Added PubMed Id

Schnabel CL, Jentsch MC, L, ü, bke S, Kaiser-Thom S, Gerber V, Vrtala S, Huang HJ, Rhyner C, Wagner B, Hoffmann R, Volke D, and yeast protein antigens in severe equine asthma in a preliminary study. Front Immunol, 14():1293684(2023) [pubmed]

submitter keyword: Immunoglobulin isotypes, proteomics, 2D immunoblot, LC-MS, asthma, COPD, RAO, heaves

Ralf Hoffmann
contact affiliation	Universität Leipzig/BBZ
contact email	ralf.hoffmann@bbz.uni-leipzig.de
lab head
Daniela Volke
contact affiliation	Universität Leipzig/BBZ
contact email	daniela.volke@bbz.uni-leipzig.de
dataset submitter

Panorama Public dataset URI