PXD045546-2

PXD045546 is an original dataset announced via ProteomeXchange.

Title	Profiling FERONIA interaction proteins by Pupylation-based interacting tagging in Arabidopsis
Description	FERONIA (FER) is a ubiquitious expressed and a plasma memberane-localized receptor-like protein kinase which modulate many biological functions. Elucidating the interacting proteins is the crucial step in understanding the molecular function of FER in regulating plant development. However, weak and transient interactions make identifying FER interacting proteins a challenge. Here, we adapted a novel proximity-based labelling technique, pupylation-based interaction tagging (PUP-IT), to label interacting proteins, subsequent enriched by affinity capture, and analyzed by liquid chromatography-coupled tandem mass spectrometery (LC-MS/MS) to profile FER interacting proteins. To perform a quick screen, a transient protoplasts expression system was conducted to identify interacting proteins in mesophyll cells. In addition, we generated a transgenic line that harboring a tagging substrate-inducible system and FER-ligase recombinant protein driven by a native promoter for surveying the interacting proteins in seedlings and flower organ. We introduce and prove the concept of PUP-IT tool designed to detect protein interactions of significance in plants.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:49.344.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045546
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhuoran Lin
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-21 00:53:14	ID requested
1	2024-08-13 03:09:12	announced
⏵ 2	2024-10-22 06:54:49	announced	2024-10-22: Updated project metadata.

10.6019/PXD045546;

submitter keyword: Arabidopsis thaliana, Orbitrap Fusion Lumos(Thermo Fisher Scientific)

Kun-hsiang Liu
contact affiliation	State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest Agriculture & Forestry University, Yangling, Shaanxi 712100, China
contact email	khliu@molbio.mgh.harvard.edu
lab head
Zhuoran Lin
contact affiliation	Northwest A&F University
contact email	805772406@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045546
     1. Label: PRIDE project
     2. Name: Profiling FERONIA interaction proteins by Pupylation-based interacting tagging in Arabidopsis