PXD045543-2

PXD045543 is an original dataset announced via ProteomeXchange.

Title	Data-independent acquisition (DIA)-MS analysis for identification of Magnaporthe oryzae secreted protein induces cell death and triggers defense responses in rice
Description	The rice blast disease, caused by Magnaporthe oryzae , devastates cultivated rice (Oryza sativa L.), resulting in extensive global crop loss. We employed a label-free quantitative proteomics approach to discover novel proteins associated with M. oryzae pathogenicity and rice defense. We identified 990 significantly modulated proteins in rice leaves including various pattern recognition receptors (PRRs) and pathogenesis-related (PR) proteins that were induced in response to M. oryzae inoculation. Additionally, 123 M. oryzae proteins were also identified and screened for their cell death-inducing activity by an in-silico approach. Among these, we found a novel protein MoXYL1 (endo-1,4-beta-xylanase) protein, which induces cell death in Nicotiana benthamiana leaves. Transgenic rice plants (PDUF26::MoXYL1) expressing MoXYL1 derived by rice domain of unknown function protein 26 (DUF26) promoter exhibited resistance against the M. oryzae and Cochliobolus miyabeanus and enhanced expression of pathogen-responsive genes and hormone-related genes. Furthermore, the application of data-independent acquisition (DIA) mass spectrometry (MS)-based proteomics on these transgenic rice plants revealed 1,833 significantly modulated proteins in response to M. oryzae, with 219 and 410 proteins responsive to MoXYL1 and M. oryzae, respectively. Based on these results, we propose a signaling network model induced by MoXYL1 and M. oryzae. In summary, our findings highlight the crucial role of MoXYL1 in rice innate immunity against M. oryzae and its potential to enhance rice disease resistance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:34:48.059.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-20 23:53:45	ID requested
1	2024-03-28 01:07:55	announced
⏵ 2	2024-10-22 06:34:48	announced	2024-10-22: Updated project metadata.

10.1111/PPL.14174;

submitter keyword: Magnaprothe oryzae, DIA-MS,Rice leaves

Sun Tae Kim
contact affiliation	Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea
contact email	stkim5505@gmail.com
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD045543

PRIDE project URI

• PRIDE
  1. PXD045543
     1. Label: PRIDE project
     2. Name: Data-independent acquisition (DIA)-MS analysis for identification of Magnaporthe oryzae secreted protein induces cell death and triggers defense responses in rice